The definitive guide to peptidomics- a hands-on lab referenceThe first truly comprehensive book about peptidomics for protein and peptide analysis, this reference provides a detailed description of the hows and whys of peptidomics and how the techniques have evolved. With chapters contributed by leading experts, it covers naturally occurring peptides, peptidomics methods and new developments, and the peptidomics approach to biomarker discovery. Explaining both the principles and the applications, Peptidomics: Methods and Applications:*Features examples of applications in di
PEPTIDOMICS; CONTENTS; Preface; Contributors; PART I STUDIES OF NATURALLY OCCURRING PEPTIDES; 1 Analysis of the Peptidomes of Amphibian Skin Granular Gland Secretions-An Integrated Functional Genomic Strategy; 1.1 Introduction; 1.2 Historical Perspective; 1.3 Contemporary Methods of Sample Acquisition; 1.4 The Integrated Functional Genomic Strategy; 1.5 How then can All Three Approaches be Integrated?; 1.6 Limitations of Each Approach; 1.7 Closing Thoughts 2 A Short History of Insect (Neuro)Peptidomics-A Personal Story of the Birth and Youth of an Excellent Model For Studying Peptidome Biology2.1 Introduction; 2.2 History; 2.3 Present (and Future): Novel Technologies, New Data. . .; 2.3.1 MALDI and (Nano)ESI TOFs and QTOFs; 2.3.2 Peptide Displays; 2.3.3 Ion Traps and FT-ICRs: Greater Variety of Instruments, Greater Variety of Sequences. . .; 2.3.4 Orbitrap; 2.3.5 FT-ICRs; 2.3.6 Combining Peptide Chemical Analysis with Histological Localization: Peptidome Mass Spectrometry Imaging; 2.4 Concluding Remarks 3 Peptidomics of Short Linear Cytolytic Peptides from Spider Venom3.1 Introduction; 3.2 Peptidomics: Identifying and Sequencing Novel AMPs; 3.3 Genomics: Unraveling the Biosynthetic Pathway; 3.4 Venomics: AMPs in the Spider Venom; 3.4.1 Structural Features; 3.4.2 Functional Features; 3.4.3 Venomic Significance; 4 Molecular Cloning Approaches to Peptidomics: The Identification of Novel cDNAs Encoding Neurotoxin-like Peptide Pools; 4.1 Introduction; 4.2 Spider Toxins-Combinatorial Peptide Libraries; 4.3 EST Cloning Approach to Cloning Peptide Families 4.4 PCR-Based Amplification and Cloning Approaches4.5 The Design and Use of Hybrid Primers for the Amplification of cDNAs Coding for Large Toxin-like Peptide Pools; 4.5.1 Multiple Alignment of the Existing Toxins and Toxin-like Sequences; 4.5.2 The Design of Hybrid Partially Degenerate Primers; 22.214.171.124 The Design of the 3 ́ Region; 126.96.36.199 The Design of the 5 ́ Region and the Middle Part of the Primers; 188.8.131.52 The Overall Length and Degeneracy; 184.108.40.206 Annealing Temperature Matching; 4.5.3 Optimization of the Amplification Conditions; 4.5.4 Other Experimental Details 4.5.5 High Throughout Amplification and Cloning4.5.6 Sequences Identification and Analysis; 4.6 Polypeptide Toxins-Novel Applications in Drug and Pesticide Development; 4.6.1 Analgesic Drugs; 4.6.2 Insecticides; 4.7 Concluding Remarks; 4.7.1 RACE-PCR Issues; 4.7.2 Sequence Quality Issues; 4.7.3 Conclusion; 5 Wheat Antimicrobial Peptides; 5.1 Introduction; 5.2 Materials and Methods; 5.2.1 Isolation of AMPs; 5.2.2 Reduction and Alkylation of Peptides ; 5.2.3 MALDI-TOF MS; 5.2.4 High-Resolution Two-Dimensional Gel Electrophoresis; 5.3 Isolation of AMPs from T. kiharae Seeds 5.4 Sequence Determination of AMPs from T. kiharae Seeds
Description based upon print version of record. Includes bibliographical references and index.