Methods in enzymology. Volume 570, Chemokines / edited by Tracy M. Handel.

First edition.
Amsterdam, [Netherlands] : Academic Press, 2016.
Methods in enzymology.
Methods in Enzymology, 0076-6879 ; Volume 570
1 online resource (676 p.)
Chemokines -- Physiological effect.
Electronic books.
Front Cover; Chemokines; Copyright; Contents; Contributors; Preface; Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface; 1. Surface Plasmon Resonance; 2. Chemokine Receptors: Members of the GPCR Family; 3. Chemokine Receptor Immobilization on the Sensor Chip; 4. Viral Particles as Chemokine Receptors Carriers in SPR; 4.1. Materials; 4.2. Method I: Generation of Retroviral Particles; 4.3. Method II: Generation of LVPs; 4.4. Method III: Titration of Viral Proteins; 4.5. Method IV: Determination of Chemokine Receptor Levels on the VP
4.6. Method V: Quantitation of Receptor Number on VPs4.7. Method VI: Attachment of VPs to Biosensor Surfaces; 5. SPR-Based Applications for Chemokine Receptors; 6. Conclusions; Acknowledgments; References; Chapter Two: Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices; 1. Introduction; 2. Methods; 2.1. Chemotaxis in Microfluidic Chemotaxis Chamber; 2.1.1. Making Microfluidic Chemotaxis Chamber; 2.1.2. Chemotaxis Assay for Differentiated HL-60 Cells; 2.2. Gradient Switching in Microfluidic Device; 2.2.1. Making a Gradient Switching Microfluidic Device
2.2.2. Chemotaxis Assay for Differentiated HL-60 Cells with Gradient Switch2.2.2.1. Cell Tracking and Data Analysis; 2.3. Cell-Cell Interaction in Tumor Microenvironment; 2.3.1. Creation of the Microfluidic Device; 3. Limitations; 4. Perspectives; 4.1. Shearing Force and Calculation of Chemotactic Force; 4.2. Cell Polarity Change Versus Cell Turning During a Gradient Change of Direction; 4.3. Potential Future Uses of Microfluidic Devices: Analysis of Circulating Tumor Cells; 4.4. Advantages of Using 3D Microbioreactors to Investigate Factors that Influence the Tumor Microenvironment
AcknowledgmentsReferences; Chapter Three: Generating Chemokine Analogs with Enhanced Pharmacological Properties Using Phage Display; 1. Introduction; 1.1. Chemokines in Health and Disease; 1.2. Chemokine Structure and Activity; 1.3. Applying Phage Display Technology to Chemokine Receptors; 2. Methods; 2.1. Library Design and Construction; 2.1.1. Choice of Phage Display System; 2.1.2. Introducing Library Diversity; 2.1.3. How Much Diversity Is Feasible?; 2.1.4. Partial Diversity; 2.1.5. Chemokine Walking: Exploring Diversity Step by Step; 2.1.6. Required Materials; 2.1.7. Library Construction
2.2. Selection of Libraries on Cells2.2.1. Considerations; 2.2.2. Cell Lines; 2.2.3. Required Materials; 2.2.4. Option 1: Selection for Internalizing Ligands; 2.2.5. Option 2: Selection for High-Affinity Cell Surface Binding; 3. Limitations; 3.1. The Atypical Chemokine Receptor DARC (ACKR1); 4. Perspectives; 4.1. A Chemokine-Based HIV Prevention Strategy; 4.2. New Tools to Study CCR5 Pharmacology and Cell Biology; 4.3. An Intrakine to Protect Cells from HIV Infection and a Vaccine Adjuvant; 4.4. A Prototypic Inhibitor of CX3CR1, the Fractalkine/CX3CL1 Receptor; References
Chapter Four: Methods for the Recognition of GAG-Bound Chemokines
Description based upon print version of record.
Includes bibliographical references at the end of each chapters and index.
Description based on online resource; title from PDF title page (ebrary, viewed March 18, 2016).
Handel, Tracy M., editor.
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