Methods in enzymology. Part B, Circadian rhythms and biological clocks / edited by Amita Sehgal ; contributors, Ravi Allada [and fifty-three others].
- First edition.
- London, England : Academic Press, 2015.
- Methods in enzymology ; Volume 552.
Methods in Enzymology, 0076-6879 ; Volume 552
1 online resource (432 p.)
- Electronic books.
- Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock fun
- Front Cover; Circadian Rhythms and Biological Clocks, Part B; Copyright; Contents; Contributors; Preface; Part I: Dissecting the Central Clock Circuit; Chapter One: Measuring Synchrony in the Mammalian Central Circadian Circuit; 1. Introduction; 1.1. What is synchrony?; 1.2. What is circadian synchrony?; 1.3. Goals of this review; 2. Monitoring SCN Rhythms with Cellular Resolution; 3. Isolating Data from Single Cells; 4. Defining a Rhythm; 4.1. Plotting rhythmic data for visual inspection; 5. Period Synchrony: Methods to Extract and Compare Periods Between Cells
5.1. Do cells share the same period?6. Phase Synchrony: Methods to Extract and Compare Phase Relationships Between Cells; 7. Perturbations Reveal Synchronization Mechanisms; 8. Methods Awaiting Application in Circadian Biology; 9. Step-by-Step Instructions for Measuring Synchrony in SCN Slice; 9.1. Bioluminescence recordings using a charge-coupled device camera; 9.2. Image processing; 9.3. Single-cell tracking; 9.4. Data presentation; Acknowledgments; References; Chapter Two: Patch-Clamp Electrophysiology in Drosophila Circadian Pacemaker Neurons; 1. The Drosophila Circadian Network
2. Circadian Control of Neuronal Activity2.1. Intrinsic currents; 2.2. Synaptic currents; 3. Methods for Patch-Clamp Electrophysiology; 3.1. Equipment; 3.2. Solutions; 3.3. Drosophila brain dissection; 3.4. Recordings; 3.5. Assessing the quality of the recording; 3.6. Relevant data in current clamp; 3.7. Relevant data in voltage clamp; 4. Conclusion; Acknowledgments; References; Chapter Three: Glial Cell Regulation of Rhythmic Behavior; 1. Introduction; 2. Studies of Glial Cell Function in Circadian Behavior and Sleep; 2.1. Glia and circadian behavior
2.2. Mammalian astrocytes and the sleep homeostat2.3. Glia-neuron communication regulates Drosophila sleep; 2.4. Microglial clocks, synaptic strength, and sleep; 3. Potential Circadian Glia-Neuron Signaling Molecules; 3.1. Glial clocks and ATP rhythms; 3.2. Glial neurotrophic factors and cytokines in circadian behavior; 3.3. Secreted molecules mediating glia-neuron communication in Drosophila; 4. Molecular Genetic Strategies for Studying the Glial Regulation of Drosophila Rhythms; 4.1. Glia-selective genetic perturbation methods
4.2. Glial expression profiling and cell-specific targeted genetic screens4.3. Glial microRNAs as regulators of rhythmicity; Appendix A. Protocol for TRAP Profiling of Fly Glial Cells; A.1. Bead preparation; A.2. Sample preparation; A.3. Immunoprecipitation; A.4. RNA extraction for RNA-seq library preparation; A.5. Reagents; References; Chapter Four: Neurophysiological Analysis of the Suprachiasmatic Nucleus: A Challenge at Multiple Levels; 1. Introduction; 2. Part I: Clock Mechanisms at the Cellular Level; 2.1. The molecular feedback loops and beyond; 2.2. Cytosolic oscillators
2.3. Role of neuropeptides
- Description based upon print version of record.
Includes bibliographical references at the end of each chapters and indexes.
Description based on online resource; title from PDF title page (ebrary, viewed March 5, 2015).
- Allada, Ravi, contributor.
Sehgal, Amita, editor.
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