RNA-ligand interactions. Part A, Structural biology methods [electronic resource] / edited by Daniel W. Celander, John N. Abelson.

San Diego, CA ; London : Academic Press, c2000.
1 online resource (583 p.)
Methods in enzymology ; v. 317.
Methods in enzymology ; v. 317

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Ligands (Biochemistry).
Electronic books.
RNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods.The critically acclaimed laboratory standard for more than forty years, Methods
Front Cover; Methods in Enzymology; Copyright Page; Table of Contents; Contributors to Volume 317; Preface; Volumes in Series; Section I: Semisynthetic Methodologies; A. RNA Synthetic Methods; Chapter 1. Advanced 5' -Silyl-2' -Orthoester Approach to RNA Oligonucleotide Synthesis; Chapter 2. Preparation of Specifically 2H- and 13C-Labeled Ribonucleotides; Chapter 3. Base-Modified Phosphoramidite Analogs of Pyrimidine Ribonucleosides for RNA Structure- Activity Studies; B. Derivatization of RNA; Chapter 4. Use of T7 RNA Polymerase and Its Mutants for Incorporation of Nucleoside Analogs into RNA
Chapter 5. Phosphorothioate Modification of RNA for Stereochemical and Interference AnalysesChapter 6. Chemical Probing of RNA by Nucleotide Analog Interference Mapping; Chapter 7. Joining of RNAs by Splinted Ligation; Chapter 8. Heavy Atom Derivatives of RNA; Chapter 9. Site-Specific Cleavage of Transcript RNA; Chapter 10. Using DNAzymes to Cut, Process, and Map RNA Molecules for Structural Studies or Modification; Section II: RNA Structure Determination; A. X-Ray Crystallography; Chapter 11. Purification, Crystallization, and X-Ray Diffraction Analysis of Small Ribozymes
Chapter 12. Solving Large RNA Structures by X-Ray CrystallographyChapter 13. Conventional and Time-Resolved Ribozyme X-Ray Crystallography; B. Nuclear Magnetic Resonance Spectroscopy; Chapter 14. Nuclear Magnetic Resonance Methods to Study RNA-Protein Complexes; Chapter 15. Filamentous Bacteriophage for Aligning RNA, DNA, and Proteins for Measurement of Nuclear Magnetic Resonance Dipolar Coupling Interactions; Chapter 16. Biochemical and Nuclear Magnetic Resonance Studies of Aminoglycoside-RNA Complexes; C. Electron Microscopy
Chapter 17. Experimental Prerequisites for Determination of tRNA Binding to Ribosomes from Escherichia coliChapter 18. Three-Dimensional Cryoelectron Microscopy of Ribosomes; Chapter 19. Preparation of Functional Ribosomal Complexes and Effect of Buffer Conditions on tRNA Positions Observed by Cryoelectron Microscopy; Section III: Techniques for Monitoring RNA Conformation and Dynamics; A. Solution Methods; Chapter 20. Probing RNA Structure and Function by Circular Permutation; Chapter 21. Kinetic Oligonucleotide Hybridization for Monitoring Kinetic Folding of Large RNAs
Chapter 22. Time-Resolved Synchrotron X-Ray Footprinting and Its Application to RNA FoldingB. Electrophoretic and Spectroscopic Methods; Chapter 23. Analysis of Global Conformation of Branched RNA Species Using Electrophoresis and Fluorescence; Chapter 24. Application of Circular Dichroism to Study RNA Folding Transitions; Chapter 25. Fluorescence Assays to Study Structure, Dynamics, and Function of RNA and RNA-Ligand Complexes; Chapter 26. Transient Electric Birefringence for Determining Global Conformations of Nonhelix Elements and Protein-Induced Bends in RNA
Section IV: Modeling Tertiary Structure
Description based upon print version of record.
Includes bibliographical references and indexes.
Celander, Daniel W. (Daniel Walter)
Abelson, John.