Franklin

Receptors [electronic resource] / edited by Ravi Iyengar, John D. Hildebrandt. Pt. B, G proteins and their regulators.

Publication:
San Diego ; London : Academic Press, c2002.
Format/Description:
Book
1 online resource (719 p.)
Series:
Methods in enzymology ; v. 343.
Methods in enzymology, 0076-6879 ; v. 343
G protein pathways ; A
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Subjects:
G proteins.
Cellular signal transduction.
Form/Genre:
Electronic books.
Language:
English
Summary:
This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the mo
Contents:
Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 343; Preface; Volume in Series; Section I: G Protein-Coupled Receptors; A. Theoretical Evaluation of Receptor Function; Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupled Receptors; Chapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of Three-State Model; B. Design and Use of Receptor Ligands; Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function
Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled ReceptorsChapter 5. Design of Peptide Agonists; Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors; Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A Receptor; C. Structural Characterization of Receptor Proteins; Chapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function of G Protein-Coupled Receptors; Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors
Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavage MethodsChapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the ß2-Adrenoceptor; Chapter 12. Crystallization of Membrane Proteins in Cubo; Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and Determining Functional Roles; Chapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin; Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin
D. Design and Use of Engineered Receptor ProteinsChapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic Ligands; Chapter 17. Analysis of Structure-Function from Expression of G Protein-Coupled Receptor Fragments; Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor-G Protein Fusion Proteins; Chapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure Function Studies of Receptors; Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors
E. Molecular Modeling Studies of Receptor Structure and FunctionChapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled Receptors by Geometric Complementarity Using the GRAMM Computer Algorithm; Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanisms; F. Analysis of Receptor Protein Coupling; Chapter 23. Reconstitution of G Protein-Coupled Receptors with Recombinant GProteinaand ß? Subunits; Chapter 24. Cell-Free Membrane Desensitization Assay for G Protein-Coupled Receptors
Chapter 25. Methods to Determine the Constitutive Activity of Histamine H2 Receptors
Notes:
Description based upon print version of record.
Includes bibliographical references and index.
Contributor:
Iyengar, Ravi.
Hildebrandt, John D.
ISBN:
1-281-01121-5
9786611011215
0-08-049692-X
OCLC:
808616160