3-D reconstruction [electronic resource] / edited by Grant J. Jensen.

1st ed.
Amsterdam : Elesevier/Academic Press, 2010.
Methods in enzymology ; v. 482.
Methods in enzymology, 0076-6879 ; v. 482
Cyro-EM ; pt. B
1 online resource (472 p.)
Electron microscopy -- Technique.
Proteins -- Structure.
Electronic books.
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. * Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to th
Front Cover; Methods in Enzymology Cryo-EM, Part B: 3-D Reconstruction; Copyright page; Contents; Contributors; Preface; Methods in Enzymology; Chapter 1: Fundamentals of Three-Dimensional Reconstruction from Projections; 1. Introduction; 2. The Object and its Projection; 3. Taxonomy of Reconstruction Methods; 4. Discretization and Interpolation; 5. The Algebraic and Iterative Methods; 6. Filtered Backprojection; 7. Direct Fourier Inversion; 8. Implementations of Reconstruction Algorithms in EM Software Packages; 9. Conclusions; Acknowledgments; References
Chapter 2: Image Restoration in Cryo-Electron Microscopy1. Introduction; 2. Image Formation Model in Electron Microscopy; 3. Estimation of Image Formation Model Characteristics; 4. Pseudoinverse Restoration-Mean-Square and Chi-Square Error Criteria; 5. Wiener Filter-the Minimum Mean-Squared Error Criterion; 6. Image Restoration for Sets of Heterogeneous Images; 7. Discussion; Acknowledgments; References; Chapter 3: Resolution Measures in Molecular Electron Microscopy; 1. Introduction; 2. Optical Resolution Versus Resolution in Electron Microscopy; 3. Principles of Resolution Assessment in EM
4. Fourier Shell Correlation and its Relation to Spectral Signal-to-Noise Ratio5. Relation Between Optical Resolution, Self-Consistency Measures, and Optimum Filtration of the Map; 6. Resolution Assessment in Electron Tomography; 7. Resolution Assessment in Practice; Acknowledgments; References; Chapter 4: 3D Reconstruction from 2D Crystal Image and Diffraction Data; 1. Introduction to Electron Crystallography Data Processing; 2. Algorithms for Electron Crystallography; 3. Image Processing with 2dx; 4. Electron Diffraction Processing with XDP; 5. Electron Diffraction Processing in IPLT
6. ConclusionsAcknowledgments; References; Chapter 5: Fourier-Bessel Reconstruction of Helical Assemblies; 1. Introduction; 2. Basic Principles; 3. Step-by-step Procedure for Helical Reconstruction; 4. Case Study of Helical Reconstruction of Ca-ATPase; 5. Conclusions; Acknowledgments; Appendix A Mathematical Foundations of Fourier-Based Helical Reconstruction; References; Chapter 6: Reconstruction of Helical Filaments and Tubes; 1. The Iterative Helical Real Space Reconstruction Approach; 2. Using IHRSR; 3. Intrinsic Ambiguities in Helical Symmetry; 4. Conclusion; Acknowledgments; References
Chapter 7: Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage1. Introduction: 3D Asymmetric Reconstruction of Tailed Bacteriophage; 2. Particle Boxing; 3. Generating a Starting Model of the Complete phi29 Phage; 4. Asymmetric Reconstruction of the Entire phi29 Phage; 5. Analysis and Interpretation of phi29 Reconstruction; 6. Summary and Future Prospects; Acknowledgments; References; Chapter 8: Single Particle Analysis at High Resolution; 1. Introduction; 2. Specimen Requirements; 3. Data Collection Requirements; 4. Introduction to EMAN; 5. Particle Selection/Boxing
6. CTF and Envelope Correction
Description based upon print version of record.
Includes bibliographical references and indexes.
Jensen, Grant J.
Location Notes Your Loan Policy
Description Status Barcode Your Loan Policy