Taxonomy of prokaryotes [electronic resource] / edited by Fred Rainey and Aharon Oren.
- 1st ed.
- London : Academic Press, 2011.
- Methods in microbiology ; v. 38.
Methods in microbiology, 0580-9517 ; v. 38
1 online resource (486 p.)
- Prokaryotes -- Classification.
- Electronic books.
- Taxonomy of Prokaryotes, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the microbiologist interested in this specialty, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology. Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbi
- Front Cover; Methods in Microbiology; Copyright Page; Dedication; Contents; Series Advisors; List of Contributors; 1. Taxonomy of Prokaryotes - Introduction; References; 2. How to Describe New Species of Prokaryotes; I. Description of New Species of Prokaryotes - A Polyphasic Approach; II. Components of the Description of a Novel Species; A. Summary/Abstract; B. Introduction; C. Methods and Materials; D. Results and Discussion; E. The Species Description; References; 3. Phenotypic and Physiological Characterization Methods; I. Introduction; II. Fundamental Physiological Tests
A. Respiratory Enzyme Tests1. Oxidase test (indophenol oxidase, cytochrome c oxidase and cytochrome a3 oxidase); 2. Catalase test; B. Relation to Oxygen; 1. Semisolid agar method; 2. Manometric method; C. Relation to Carbon Dioxide; 1. Manometric method (Han et al., 1991; Smibert and Krieg, 1994); 2. Methods for autotrophs; D. Relation to Hydrogen; 1. Manometric method for the heterotroph Campylobacter mucosalis; 2. Manometric method for Herbaspirillum autotrophicum; E. Temperature Range and Optima for Growth; F. Optimum pH and pH Range for Growth
G. NaCl or Seawater Ranges and Optima for GrowthH. Oxidative and Fermentative Metabolism; 1. O/F test; I. Acid Production from Carbohydrates; J. Gas Production from Carbohydrates; K. Fermentation Products; 1. Capillary GLC method; 2. HPLC method 1; 3. HPLC method 2; L. Carbon Source Utilization; 1. Auxanography (the use of a plate culture in which variable conditions are provided to determine the effect of these conditions on growth); 2. Turbidimetric method; M. Nitrogen Source Utilization; 1. Sole nitrogen sources; 2. Nitrogen fixation; N. Nitrate Reduction and Denitrification
1. Nitrate reduction2. Nitrite reduction; 3. Detection of N2O as evidence of denitrification; O. Hydrolysis of Polymers; 1. Agar hydrolysis; 2. AzocollTM protein hydrolysis; 4. DNA hydrolysis; 5. Gelatin and casein hydrolysis; 6. Starch hydrolysis; P. Pigmentation; 1. Fluorescent pigments; 2. Diffusible, non-fluorescent pigments; 3. Water-soluble pigments from aromatic amino acids; 4. Water-insoluble pigments; Q. Iron Porphyrin Compounds; III. Other Physiological Tests; A. Acetamide Hydrolysis; B. Ammonia from Arginine; C. Aromatic Ring Cleavage; 1. p-Nitrophenyl sulfate method
N. Indoxyl Acetate Hydrolysis
- Description based upon print version of record.
Includes bibliographical references and index.
- Rainey, Fred A.
Oren, Aharon, 1945-
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