The zebrafish [electronic resource] : cellular and developmental biology / edited by H. William Detrich III, Monte Westerfield, Leonard I. Zon.

Other Title:
Zebrafish: 2nd edition, cellular and developmental biology
2nd ed.
Amsterdam ; Boston : Elsevier Academic Press, c2004.
Methods in cell biology ; v. 76.
Methods in cell biology ; 76
1 online resource (655 p.)
Zebra danio -- Physiology.
Zebra danio -- Development.
Zebra danios as laboratory animals.
Cytology -- Methodology.
Electronic books.
This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.* Details state-of-the art zebra
Front Cover; The Zebrafish: Cellular and Developmental Biology; Copyright Pagae; Contents; Contributors; Preface; Dedication; Part I: Cell Biology; Chapter 1. Use of the DsRed Fluorescent Reporter in Zebrafish; I. Introduction; II. DsRed: History and Properties; III. DsRed in Transgenic Zebrafish; IV. Use of Multiple Fluorescent Reporters in Transplantation Assays; V. Fusion Protein Reporters; VI. Conclusion; References; Chapter 2. Analyzing Axon Guidance in the Zebrafish Retinotectal System; I. Introduction; II. Retinotectal Mutants; III. Labeling the Retinotectal System
IV. Perturbing the Retinotectal SystemV. Future Directions; References; Chapter 3. Optical Projection Tomography for Spatio-Temporal Analysis in the Zebrafish; I. Introduction; II. The Principle of Tomography; III. Problems for OPT in Zebrafish; IV. Materials; V. Methods; VI. Sample Preparation; VII. Scanning; VIII. Reconstruction; IX. Presentation of Reconstructions; X. Discussion; XI. The Future of OPT; XII. OPT Equipment; References; Chapter 4. Imaging Blood Vessels in the Zebrafish; I. Introduction; II. Imaging Vascular Gene Expression; III. Nonvital Blood Vessel Imaging
IV. Vital Imaging of Blood VesselsV. Conclusion; References; Chapter 5. Zebrafish Apoptosis Assays for Drug Discovery; I. Introduction; II. Zebrafish Apoptosis Genes; III. Caspase Substrate Recognition in Zebrafish; IV. Detection of Apoptosis In Situ; V. Perspectives; References; Chapter 6. Lipid Metabolism in Zebrafish; I. Introduction; II. Genetic Screen-Forward, Reverse, and Targeted-Mutagenesis; III. Pharmacological Studies Using Zebrafish; IV. Mechanism of Action Studies Using Zebrafish. The Annexin2-Caveolin1 Complex; V. Gene-Specific Studies: The Acyl-CoA Synthetase Enzymes
VI. SummaryReferences; Chapter 7. Analysis of the Cell Cycle in Zebrafish Embryos; I. Introduction: The Cell Cycle in Zebrafish; II. Zebrafish Embryo Cell Cycle Protocols; III. Screening for Mutations Affecting the Zebrafish Cell Cycle; IV. Screening for Chemical Suppressors of Zebrafish Cell Cycle Mutants; V. Conclusions; VI. Reagents and Supplies; References; Chapter 8. Cellular Dissection of Zebrafish Hematopoiesis; I. Introduction; II. Zebrafish Hematopoiesis; III. Hematopoietic Cell Transplantation; IV. Enrichment of Hematopoietic Stem Cells; V. Conclusions; References
Chapter 9. Culture of Embryonic Stem Cell Lines from ZebrafishI. Introduction; II. Methods; III. Materials; References; Part II: Developmental and Neural Biology; Chapter 10. Neurogenesis; I. Introduction; II. The Primary Neuronal Scaffold; III. Early Development of the Zebrafish Neural Plate; IV. Lateral Inhibition and the Neurogenesis Cascade; V. Establishment of Neuronal Identity; VI. Aspects of Neuronal Differentiation; VII. Useful Tools for the Study of Zebrafish Neurogenesis; VIII. Conclusion; References; Chapter 11. Time-Lapse Microscopy of Brain Development
I. Introduction-Why and When to Use Intravital Imaging
Description based upon print version of record.
Includes bibliographical references and index.
Detrich, H. William.
Westerfield, Monte.
Zon, Leonard I.
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