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Proteomics [electronic resource] : Methods and Protocols / edited by Lucio Comai, Jonathan E. Katz, Parag Mallick.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2017.
Format/Description:
Book
1 online resource (XII, 371 p.) : 64 illus., 48 illus. in color.
Series:
Methods in molecular biology 1064-3745 ; 1550
Methods in Molecular Biology, 1064-3745 ; 1550
Contained In:
Springer eBooks
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Details

Subjects:
Life sciences.
Proteins.
System Details:
text file PDF
Summary:
This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
A Robust Protocol for Protein Extraction and Digestion
Improving Proteome Coverage and Sample Recovery with Enhanced FASP (Efasp) For Quantitative Proteomic Experiments
Proteome Characterization of a Chromatin Locus using the Proteomics of Isolated Chromatin Segments Approach
Profiling Cell Lines Nuclear Sub-Proteome
Optimized Enrichment of Phosphoproteomes by Fe-IMAC Column Chromatography
Full Membrane Protein Coverage Digestion And Quantitative Bottom-Up Mass Spectrometry Proteomics
Hydrophilic Strong Anion Exchange (Hsax) Chromatography Enables Deep Fractionation Of Tissue Proteomes
High Ph Reversed-Phase Micro-Columns for Simple, Sensitive and Efficient Fractionation of Proteome and (TMT-Labeled) Phosphoproteome Digests
Multi-Lectin Affinity Chromatography for Separation, Identification, and Quantitation of Intact Protein Glycoforms in Complex Biological Mixtures
Parallel Exploration of Interaction Space by Bioid and Affinity Purification Coupled to Mass Spectrometry
LUMIER: A Discovery Tool for Mammalian Protein Interaction Networks
Dual Color, Multiplex Analysis of Protein Microarrays for Precision Medicine
Quantitative Proteomics Using SILAC
Relative Protein Quantification using Tandem Mass Tag Mass Spectrometry
Pathway-Informed Discovery and Targeted Proteomic Workflows Using Mass Spectrometry
Generation of High Quality SWATH Acquisition Data for Label-Free Quantitative Proteomics Studies Using Tripletof® Mass Spectrometers
Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols
Protein Micropatterning Assay - Quantitative Analysis of Protein-Protein Interactions
Designing Successful Proteomics Experiments
Automated SWATH Data Analysis using Targeted Extraction of Ion Chromatograms
Virtualization of Legacy Instrumentation Control Computers for Improved Reliability, Operational Life, and Management
Statistical Assessment of QC Metrics on Raw LC-MS/MS Data
Data Conversion with proteoWizard msConvert.
Contributor:
Comai, Lucio. editor.
Katz, Jonathan E. editor.
Mallick, Parag. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781493967476
Publisher Number:
10.1007/978-1-4939-6747-6 doi
Access Restriction:
Restricted for use by site license.