Amyloid Proteins [electronic resource] : Methods and Protocols / edited by Einar M. Sigurdsson, Miguel Calero, María Gasset.

3rd ed. 2018.
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Methods in Molecular Biology, 1064-3745 ; 1779
Methods in Molecular Biology, 1064-3745 ; 1779
1 online resource (XVII, 547 pages) : 99 illustrations, 65 illustrations in color.
Life sciences.
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Life Sciences.
Protein Science.
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This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.
Preparation of Pure Populations of Amyloid-β Protein Oligomers of Defined Size
Preparation of Well-Defined and Stable β-Barrel Pore-Forming Amyloid-β42 Oligomers
Unveiling Brain Aβ Heterogeneity through Targeted Proteomic Analysis
Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments
Generation and Characterization of Stable α-Synuclein Oligomers
In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity
Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices
Purification and Characterization of Low-n Tau Oligomers
Preparation and Characterization of Tau Oligomer Strains
Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein and Tau under Native Conditions
Preparation of Amyloidogenic Aggregates from EF-Hand β -parvalbumin and S100 Proteins
Kinetic Analysis of Amyloid Formation
Mapping Amyloid Regions in Gad m 1 with Peptide Arrays
Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering
Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation
Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion
Bacterial Amyloids
Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein
Study of Amyloids Using Yeast
Neurotoxic Ca2+ Signaling Induced by Amyloid-β Oligomers in Aged Hippocampal Neurons In Vitro
Preparation and Culturing of Human Primary Vascular Cells
Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model
Effects of Amyloid-β Peptide on the Biology of Human Neural Stem Cells
Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes
Identification and Characterization of Amyloid-β Accumulation in Synaptic Mitochondria
Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates
Tau Assembly into Filaments
Quantitative Metabolomics in Alzheimer´s Disease: Technical Considerations for Improved Reproducibility
Detecting Circulating MicroRNAs as Biomarkers in Alzheimer's Disease
Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues
Characterization of Amyloid-β Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer´s Disease Brain: A Confocal Microscopy Approach
In Vivo Imaging of Tauopathy in Mice
In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI.
Sigurdsson, Einar M., editor.
Calero, Miguel, editor.
Gasset, Maria, editor.
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