Franklin

In Situ Hybridization Methods [electronic resource] / edited by Giselbert Hauptmann.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2015.
Series:
Neuromethods 0893-2336 ; 99
Springer Protocols (Springer-12345)
Neuromethods, 0893-2336 ; 99
Format/Description:
Book
1 online resource (XX, 592 pages) : 109 illustrations, 94 illustrations in color.
Subjects:
Neurosciences.
Neurobiology.
Local subjects:
Neurosciences. (search)
Neurobiology. (search)
System Details:
text file PDF
Summary:
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.   Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
Contents:
Optimization of In Situ Hybridization and Co
Expression Analysis in the Central Nervous System
In Situ Hybridization Methods for RNA Visualization in C. elegans
Multicolored Visualization of Transcript Distributions in Drosophila Embryos
Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) in Whole-Mount Embryos
Exploring Brain Genoarchitecture by Single and Double Chromogenic In Situ Hybridization (ISH) and Immunohistochemistry (IHC) on Cryostat, Paraffin, or Floating Sections
Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH)
One FISH, dFISH, three FISH:  Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians
Whole-Mount In Situ Hybridization and Immunohistochemistry in Xenopus Embryos
Manual and Automated Whole-Mount In Situ Hybridization for Systematic Gene Expression Analysis in Embryonic Zebrafish Forebrain
Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse Diencephalon
High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond
Analysis of Embryonic Gene Expression Patterns in the Hagfish
Gene Expression Analysis of Lamprey Embryos
Detection of mRNA and MicroRNA Expression in Basal Chordates, Amphioxus and Ascidians
Analysis of Gene Expression in Neurons and Synapses by Multi-Color In Situ Hybridization
Fluorescent In Situ Hybridization in Primary Hippocampal Neurons to Detect Localized mRNAs
Combining Whole-Mount In Situ Hybridization with Neuronal Tracing and Immunohistochemistry
Fluorescent In-Situ Hybridization Detection of MicroRNAs in Newt Tissue Cryosections
Subcellular Transcript Localization in Drosophila Embryos and Tissues Visualized by Multiplex FISH
Application of Alkaline Phosphatase-Mediated Azo Dye Staining for Dual Fluorescent In Situ Hybridization in Zebrafish
Multiplex Fluorescent RNA In Situ Hybridization via RNAscope
Quantum Dot-Based In Situ Hybridization and Immunohistochemistry to Detect mRNA and Protein at Subcellular Levels, Comparison with Studies under Electron Microscopy
Quantitative Multiplexed Quantum Dot Based In Situ Hybridisation in Formalin Fixed Paraffin Embedded Tissue
Single and Multiplexed Fluorescent In Situ Hybridization using Quantum Dots Nanocrystals in Xenopus
Quantitative Single Molecule mRNA Fluorescent In Situ Hybridization in C. elegans
In Situ Detection of Individual mRNA Molecules with Padlock Probes and Target-Primed Rolling-Circle Amplification in Fixed Mouse Brain Tissues
Extended Multiplexed Fluorescent In Situ Hybridisation by Combinatorial Encoding of Individual Transcripts
Characterizing Cellular Identity at One Cell Resolution
Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues
ECHO-FISH for Gene Transcript Detection in Neuronal and Other Cells and Subcellular Compartments.
Contributor:
Hauptmann, Giselbert. editor., Editor,
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
Printed edition:
Printed edition:
ISBN:
9781493923038
Publisher Number:
10.1007/978-1-4939-2303-8 doi
Access Restriction:
Restricted for use by site license.
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