Estrogen Receptors [electronic resource] : Methods and Protocols / edited by Kathleen M. Eyster.

New York, NY : Springer New York : Imprint: Humana Press, 2016.
Methods in molecular biology 1064-3745 ; 1366
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1366
1 online resource (XVI, 576 pages) : 129 illustrations.
Life sciences.
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This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen. .
The Estrogen Receptors: An Overview from Different Perspectives
Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER)
The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression
Bioinformatics Analysis of Estrogen-Responsive Genes
Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen
Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors
Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells
Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer
RNA-Seq Experiment and Data Analysis
DNA Microarray Analysis of Estrogen-Responsive Genes
Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry
Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen
Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy
Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy
In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis
Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a
Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction
Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy
Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin
Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor α and Specificity Protein 1 with Composite DNA Elements
Tips and Tricks for Successful Application of Statistical Methods to Biological Data
The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites
Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model
Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture
Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay
Silencing Estrogen Receptor-α with siRNA in the Intact Rodent Brain
Silencing Estrogen Receptor-β with siRNA in Cultured Cells
Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists
Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells
Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR.-Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils
Estrogen Receptor-α Knockout Mice
Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol
Phytoestrogens Activate the Estrogen Receptor in HepG2 Cells
Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation
GPER Mediates Non-Genomic Effects of Estrogen
GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism
Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation
Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods
Assessing Direct Vascular Actions of Estrogens
Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERα
Regulation of Activation Induced Deaminase (AID) by Estrogen.
Eyster, Kathleen M. editor.
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