Fluorescence in situ Hybridization (FISH) [electronic resource] : Protocols and Applications / edited by Joanna M. Bridger, Emanuela V. Volpi.
- Totowa, NJ : Humana Press : Imprint: Humana Press, 2010.
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 659
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 659
1 online resource (XIV, 451 pages)
- Life sciences.
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- Life Sciences.
Animal Genetics and Genomics.
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- The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.
- Core Techniques
Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology
Fluorescence In Situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibres
Detection of Nascent RNA Transcripts by Fluorescence In Situ Hybridization
Fluorescence In Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays
Fluorescence in situ Hybridization (FISH) on Tissue Cryosections
Multiplex Fluorescence in situ Hybridization (M-FISH)
Optical Mapping of Protein-DNA Complexes on Chromatin Fibers
3D-FISH on Cultured Cells Combined with Immunostaining
Technical Advancements and Novel Adaptations
The Comet-FISH Assay for the Analysis of DNA Damage and Repair
Direct In Situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes
LNA-FISH for Detection of MicroRNAs in Frozen Sections
Chromosome Orientation Fluorescence In Situ Hybridization or Strand-Specific FISH
Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells
Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation
CryoFISH: Fluorescence In Situ Hybridization on Ultrathin Cryosections
Characterization of Chromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)
Visualizing Nucleic Acids in Living Cells by Fluorescence In Vivo Hybridization
Translational FISH: Applications for Human Genetics and Medicine
Quality Control in FISH as Part of a Laboratory's Quality Management System
FlashFISH: "Same Day" Prenatal Diagnosis of Common Chromosomal Aneuploidies
FISH for Pre-implantation Genetic Diagnosis
PNA-FISH on Human Sperm
POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism
Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)
ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material
Fluorescence In Situ Hybridization on 3D Cultures of Tumor Cells
Simultaneous Ultrasensitive Subpopulation Staining/Hybridization In Situ (SUSHI) in HIV-1 Disease Monitoring
Protocols for Model Organisms
Detection of Prokaryotic Cells with Fluorescence In Situ Hybridization
FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast
FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata
Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic Heterochromatin of Drosophila
Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences
Fluorescence in situ Hybridization (FISH) for Genomic Investigations in Rat
Fluorescence In Situ Hybridization on Early Porcine Embryos
FISH on 3D Preserved Bovine and Murine Preimplantation Embryos.
- Bridger, Joanna M. editor.
Volpi, Emanuela V. editor.
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- 10.1007/978-1-60761-789-1 doi
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- Restricted for use by site license.
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