Chemotaxis [electronic resource] : Methods and Protocols / edited by Tian Jin, Dale Hereld.

Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
1 online resource (XVI, 538 pages) : 132 illustrations, 5 illustrations in color.
Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 571
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 571
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Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.
Microfluidic Techniques for the Analysis of Bacterial Chemotaxis
Prokaryotic Phototaxis
Photoorientation in Photosynthetic Flagellates
Dictyostelium Slug Phototaxis
Electrotaxis and Wound Healing: Experimental Methods to Study Electric Fields as a Directional Signal for Cell Migration
Chemotropism During Yeast Mating
Group Migration and Signal Relay in Dictyostelium
Quantitative Analysis of Distal Tip Cell Migration in C. elegans
Inflammation and Wound Healing in Drosophila
Neutrophil Motility In Vivo Using Zebrafish
Chemotaxis in Neutrophil-Like HL-60 Cells
Chemokine Receptor Dimerization and Chemotaxis
Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Breast Cancer Cell Movement: Imaging Invadopodia by TIRF and IRM Microscopy
In Vivo Assay for Tumor Cell Invasion
Quantitative Studies of Neuronal Chemotaxis in 3D
Assays for Chemotaxis and Chemoattractant-Stimulated TorC2 Activation and PKB Substrate Phosphorylation in Dictyostelium
Biochemical Responses to Chemoattractants in Dictyostelium: Ligand-Receptor Interactions and Downstream Kinase Activation
Quantifying In Vivo Phosphoinositide Turnover in Chemotactically Competent Dictyostelium Cells
In Vivo Measurements of Cytosolic Calcium in Dictyostelium discoideum
Chemokine Receptor Signaling and HIV Infection
Spatiotemporal Stimulation of Single Cells Using Flow Photolysis
Spatiotemporal Regulation of Ras-GTPases During Chemotaxis
FRAP Analysis of Chemosensory Components of Dictyostelium
Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging, and Single-Molecule Imaging
Imaging Actin Cytoskeleton Dynamics in Dictyostelium Chemotaxis
Analysis of Actin Assembly by In Vitro TIRF Microscopy
Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells
Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
Light Microscopy to Image and Quantify Cell Movement
Mathematics of Experimentally Generated Chemoattractant Gradients
Modeling Spatial and Temporal Dynamics of Chemotactic Networks
Computational Modeling of Signaling Networks for Eukaryotic Chemosensing.
Jin, Tian. editor.
Hereld, Dale. editor.
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