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Strain Engineering [electronic resource] : Methods and Protocols / edited by James A. Williams.

Publication:
Totowa, NJ : Humana Press, 2011.
Format/Description:
Book
1 online resource (XI, 480 pages) : 83 illustrations, 2 illustrations in color.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 765
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 765
Contained In:
Springer eBooks
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Subjects:
Medicine.
Human genetics.
Genetic engineering.
Local subjects:
Biomedicine. (search)
Human Genetics. (search)
Genetic Engineering. (search)
System Details:
text file PDF
Summary:
Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.
Contents:
Bacterial Genome Reengineering
Targeted Chromosomal Gene Knockout using PCR Fragments
Scarless Chromosomal Gene Knockout Methods
Random Chromosomal Gene Disruption In vivo using Transposomes
Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection
Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries
Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements
Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli
Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli
Assembling New E. coli Strains by Transduction using Phage P1
Yeast Bioinformatics and Strain Engineering Resources
Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae
Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans
Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries
Global Strain Engineering by Mutant Transcription Factors
Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae
Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources
Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria
Broad-host-range Plasmid Vectors for Gene Expression in Bacteria
Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome
Transposon-mediated Random Mutagenesis of Bacillus subtilis
Integrative Food Grade Expression System for Lactic Acid Bacteria
ClosTron-mediated Engineering of Clostridium
High Throughput Transposon Mutagenesis of Corynebacterium glutamicum
Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis
Engineering Thermoacidophilic Archaea using Linear DNA Recombination
Targeted Gene Disruption in Koji Mold Aspergillus oryzae
Selectable and Inheritable Gene Silencing through RNA Interference in the Unicellular Alga Chlamydomonas reinhardtii.
Contributor:
Williams, James A. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781617791970
Publisher Number:
10.1007/978-1-61779-197-0 doi
Access Restriction:
Restricted for use by site license.