Franklin

Pichia Protocols [electronic resource] / edited by James M. Cregg.

Edition:
Second Edition.
Publication:
Totowa, NJ : Humana Press, 2007.
Series:
Methods in Molecular Biology, 1064-3745 ; 389
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 389
Format/Description:
Book
1 online resource (XII, 268 pages)
Subjects:
Chemistry.
Biotechnology.
Cytology.
Microbial genetics.
Microbial genomics.
Microscopy.
Local subjects:
Chemistry.
Biotechnology.
Cell Biology.
Microbial Genetics and Genomics.
Biological Microscopy.
System Details:
text file PDF
Summary:
The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system.
Contents:
Vectors and Strains for Expression
DNA-Mediated Transformation
Rational Design and Optimization of Fed-Batch and Continuous Fermentations
Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1)
Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C
Rapid Screening of Chromatography Resins for the Purification of Proteins
Characterization of O-Linked Saccharides on Glycoproteins
Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids
N-Linked Glycan Characterization of Heterologous Proteins
Heavy Labeling of Recombinant Proteins
Selenomethionine Labeling of Recombinant Proteins
Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
Classical Genetics
Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration
Characterization of Protein-Protein Interactions
Localization of Proteins and Organelles Using Fluorescence Microscopy
Fluorescence Microscopy and Thin-Section Electron Microscopy.
Contributor:
Cregg, James M. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781597454568
Publisher Number:
10.1007/978-1-59745-456-8 doi
Access Restriction:
Restricted for use by site license.
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