Single Nucleotide Polymorphisms [electronic resource] : Methods and Protocols / edited by Pui-Yan Kwok.
- Totowa, NJ : Springer New York, 2003.
- Methods in Molecular Biology™, 1064-3745 ; 212
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 212
1 online resource (XI, 269 pages) : 42 illustrations, 18 illustrations in color.
- Local subjects:
- Biomedicine. (search)
Human Genetics. (search)
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- text file PDF
- Single nucleotide polymorphisms (SNPs) have become the markers of choice in elucidating the relationship between DNA sequence variation and susceptibility to disease and have clearly become the focus of the next phase of the human genome project. In Single Nucleotide Polymorphisms: Methods and Protocols, Pui-Yan Kwok, MD, PhD, has assembled a collection of robust techniques for the difficult process of SNP discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection. Described in step-by-step detail by their inventors, each method provides extensive notes on the technical steps critical for experimental success, time-saving techniques, and tips on avoiding pitfalls. Comprehensive and authoritative, Single Nucleotide Polymorphisms: Methods and Protocols provides in a readily reproducible format all the major SNP discovery and genotyping techniques in use today, whether for using DNA diagnostics to identify a pathogen, for studying the genetic basis of human disease, or for molecular breeding programs in agriculture.
- SNPs: Why Do We Care?
Denaturing High-Performance Liquid Chromatography
SNP Detection and Allele Frequency Determination by SSCP
Conformation-Sensitive Gel Electrophoresis
Detection of Mutations in DNA by Solid-Phase Chemical Cleavage Method
SNP Discovery by Direct DNA Sequencing
Computational SNP Discovery in DNA Sequence Data
Genotyping SNPs With Molecular Beacons
SNP Genotyping by the ?'-Nuclease Reaction
Genotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide Microarrays
Quantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase Minisequencing
Homogeneous Primer Extension Assay With Fluorescence Polarization Detection
Pyrosequencing for SNP Genotyping
Homogeneous Allele-Specific PCR in SNP Genotyping
Oligonucleotide Ligation Assay
Invader Assay for SNP Genotyping
MALDI-TOF Mass Spectrometry-Based SNP Genotyping.
- Kwok, Pui-Yan. editor.
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- Publisher Number:
- 10.1385/1592593275 doi
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