In Vitro Toxicity Testing Protocols [electronic resource] / edited by Sheila O'Hare, Chris K. Atterwill.

Totowa, NJ : Humana Press, 1995.
1 online resource (XIV, 332 pages) : 10 illustrations.
Methods in Molecular Biology™, 1064-3745 ; 43
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 43
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In vitro toxicology is one of the most rapidly expanding areas of biological research today. It is generally conceded that this is a result of pressure from the public for safer products and environmental conditions and, in these recessionary times, pressure from company accountants, who often perceive in vitro experimentation as a cheaper option than lawsuits, fines, and expensive remediation. Toxicologists themselves form one group whose vigor and influence on the evolution of in vitro toxicology is often underestimated. Many toxicologists have for some time been unhappy with the quantity (often a result of unnecessary duplication), the reliability, and the ethical circumstances of much animal experimentation. It is hoped that the present book, In Vitro Toxicity Testing Protocols, will play some part in enabling and encouraging the more widespread use of in vitro techniques. Since in vitro toxicity testing methodology is evolving at such a rapid rate, this book can of necessity provide only a "snapshot" of the major techniques in use at the present time. As with the other publications in Humana Press' Methods in Molecular Biology series, the aim has been to supply information on the fundamental requirements for the establishment of new techniques within working laboratories and to describe a variety of basic in vitro methodologies. Certain of the techniques detailed here could more accurately be described as ex vivo rather than truly in vitro. No apology is made for the inclusion of these techniques.
Alternative Method of Assessing Toxicity
Alternative Method of Assessing Toxicity
Target Organ Toxicity
LLC-RK1 Cell Screening Test for Nephrotoxicity
Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity
Human Thyroid Culture
Thyroid Follicular Cells in Monolayer Culture
Dust Toxicity in Rat Alveolar Macrophage Cultures
Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity
Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepatocytes
Model Cavity Method
Human Esophageal Culture
The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing
The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing
General and Topical Toxicity
The FRAME Cytotoxicity Test (Kenacid Blue)
Allium Test
Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose
MTT Assays
V79 Cytotoxicity Test for Membrane Damage
SIRC Cytotoxicity Test
Rabbit Articular Chondrocyte Functional Toxicity Test
Balb/c 3T3 Cytotoxicity Test
The Pollen Tube Growth Test
Irritancy Testing
The Use of the Bovine Isolated Cornea as a Possible In Vitro Test for Occular Irritancy
Cytotoxicity in an Anchorage-Independent Fibroblast Cell Line Measured by a Combination of Fluorescent Dyes
Cell Culture Phototoxicity Test
The Murine Local Lymph Node Assay
Polymorphonuclear Leukocyte Locomotion
Immune Function Assays
The DNA Alkaline Unwinding Genotoxicity Test
Measurement of Unscheduled DNA Synthesis In Vitro Using Primary Rat Hepatocyte Cultures
Gene Mutation Assays in Mammalian Cells
Measurement of Chromosome Aberrations In Vitro Using Human Peripheral Blood Lymphocytes
Mutation Assays in Bacteria
Submammalian and Subvertebrate Models
Chick Embryotoxicity Screening Test (CHEST I and II)
Frog Embryo Teratogenesis Assay
The Drosophila melanogaster Assay
The Hydra attenuata Assay.
O'Hare, Sheila. editor.
Atterwill, C. K., editor.
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