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High-Throughput Phenotyping in Plants [electronic resource] : Methods and Protocols / edited by Jennifer Normanly.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2012.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 918
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 918
Format/Description:
Book
1 online resource (XI, 365 pages) : 60 illustrations, 46 illustrations in color.
Subjects:
Life sciences.
Botany.
Plant genetics.
Local subjects:
Life Sciences.
Plant Sciences.
Plant Genetics & Genomics.
System Details:
text file PDF
Summary:
Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High-Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, High-Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.
Contents:
Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis
High Throughput Phenotyping of Plant Shoots
High Throughput Phenotyping of Root Growth Dynamics
LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms
Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach
Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content
High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field
High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant
High Throughput Fractionation of Natural Products for Drug Discovery
Conducting Molecular Biomarker Discovery Studies in Plants
Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry
Qualitative and Quantitative Screening of Amino Acids in Plant Tissues
Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis
ICP-MS as a Tool For High Throughput Analysis of Plants
The Plant Volatilome: Methods of Analysis
High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry
Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta
A High Throughput Biological Conversion Assay for Determining Lignocellulosic Quality
Carbohydrate Microarrays in Plant Science.
Contributor:
Normanly, Jennifer. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781617799952
Publisher Number:
10.1007/978-1-61779-995-2 doi
Access Restriction:
Restricted for use by site license.
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