Hypertension [electronic resource] : Methods and Protocols / edited by Jérôme P. Fennell, Andrew H. Baker.

Totowa, NJ : Humana Press, 2005.
Methods In Molecular Medicine™, 1543-1894 ; 108
Springer Protocols (Springer-12345)
Methods In Molecular Medicine™, 1543-1894 ; 108
1 online resource (XVI, 502 pages) : 98 illustrations.
Local subjects:
Medicine & Public Health. (search)
Cardiology. (search)
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Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.
Models Of Hypertension
Congenic/Consomic Models of Hypertension
Mouse Knockout Models of Hypertension
Production of Transgenic Models in Hypertension
Measuring Blood Pressure in Small Laboratory Animals
Assessment Of Free Radicals In Endothelial Dysfunction
Analysis of Superoxide Anion Production in Tissue
Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence
A Guide to Wire Myography
Nucleic Acid Techniques
Selection of Candidate Genes in Hypertension
Genome-Wide Scanning With SSLPs in the Rat
Extraction of RNA From Cells and Tissue
Single-Strand Conformational Polymorphism Analysis
Single-Nucleotide Polymorphism Genotyping for Disease Association Studies
Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System
TaqMan Real-Time PCR Quantification Conventional and Modified Methods
Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR
New Advances in Microarrays Finding the Genes Causally Involved in Disease
Pharmacogenetics and Pharmacogenomics
Proteins And Proteomics
Determination of the Endogenous Nitric Oxide Synthase Inhibitor Asymmetric Dimethylarginine in Biological Samples by HPLC
Proteomic Approaches in the Analysis of Hypertension
Gene Transfer
Gene Transfer in Endothelial Dysfunction and Hypertension
Nonviral Gene Delivery Methods in Cardiovascular Diseases
Construction and Characterization of Helper-Dependent Adenoviral Vectors for Sustained In Vivo Gene Therapy
Adenovirus-Mediated Gene Transfer In Vivo An Approach to Reduce Oxidative Stress
Gene Therapy for Hypertension
Modulation of Gene Expression by RNAi
In Vivo Biopanning
Stem Cells
Cardiomyocytes Derived From Embryonic Stem Cells
In Silico and Wet-Bench Identification of Nuclear Matrix Attachment Regions
Biomedical Informatics Methods in Pharmacogenomics.
Fennell, Jérôme P. editor.
Baker, Andrew H. editor.
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10.1385/1592598501 doi
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