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Plant Signal Transduction [electronic resource] : Methods and Protocols / edited by Jose R. Botella, Miguel A. Botella.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2016.
Format/Description:
Book
1 online resource (XV, 241 pages) : 41 illustrations, 25 illustrations in color.
Edition:
2nd ed. 2016.
Series:
Methods in molecular biology 1064-3745 ; 1363
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1363
Contained In:
Springer eBooks
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Details

Subjects:
Life sciences.
Botany.
Local subjects:
Life Sciences. (search)
Plant Sciences. (search)
System Details:
text file PDF
Summary:
This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of 'classic' as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.
Contents:
Suppressor Screens in Arabidopsis
Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants
DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants
Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology
Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation
Culture of the Tomato Micro-Tom Cultivar in Greenhouse
Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications
Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins
Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions
Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana
Analysis of Protein-Lipid Interactions Using Purified C2 Domains
Assessing Kinase Activity in Plants with In-Gel Kinase Assays
Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions
Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus
Simultaneous Determination of Plant Hormones by GC-TOF-MS.
Contributor:
Botella, Jose R. editor.
Botella, Miguel A. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781493931156
Publisher Number:
10.1007/978-1-4939-3115-6 doi
Access Restriction:
Restricted for use by site license.