Franklin

MHC Protocols [electronic resource] / edited by Stephen H. Powis, Robert W. Vaughan.

Publication:
Totowa, NJ : Humana Press, 2003.
Series:
Methods in Molecular Biology™, 1064-3745 ; 210
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 210
Format/Description:
Book
1 online resource (XII, 340 pages)
Subjects:
Medicine.
Immunology.
Local subjects:
Biomedicine. (search)
Immunology. (search)
System Details:
text file PDF
Summary:
Many of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease association and play a pivotal role in the control of immune response. In MHC Protocols, leading experts detail readily reproducible methods for identifying these polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP2, C2, C4, and TNF-a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is written by an investigator who has used the technique extensively and includes troubleshooting tips and notes on pitfalls to avoid. Comprehensive and highly practical, MHC Protocols offers both experienced and novice investigators not only a state-of-the-art overview of this dramatically evolving area of immunology, but also a reliable guide to the identification of MHC gene polymorphisms.
Contents:
Databases
HLA Informatics
Accessing HLA Sequencing Data Through the 6ace Database
Polymorphism in Classical and Nonclassical Hla Genes
HLA Typing by Restriction Fragment Length Polymorphism Analysis
PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles
PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR
HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes
PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles
HLA Typing With Reference Strand-Mediated Conformation Analysis
Sequencing Protocols for Detection of HLA Class I Polymorphism
HLA-E and HLA-G Typing
Typing Alleles of HLA-DM
Polymorphism in Non-Hla Mhc Genes
Typing Alleles of TAP1 and TAP2
Determining Alleles of the C2 Gene by Southern Blotting
Complement C4 Protein and DNA Typing Methods
Typing of Tumor Necrosis Factor Alleles
Molecular Typing of the MHC Class I Chain-Related Gene Locus
Microsatellites
HLA Microsatellite Analysis.
Contributor:
Powis, Stephen H. editor.
Vaughan, Robert W. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781592592913
Publisher Number:
10.1385/1592592910 doi
Access Restriction:
Restricted for use by site license.
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