Franklin

Hepatitis C [electronic resource] : Methods and Protocols / edited by Hengli Tang.

Publication:
Totowa, NJ : Humana Press, 2009.
Series:
Methods in Molecular Biology™, 1064-3745 ; 510
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 510
Format/Description:
Book
1 online resource (XXII, 466 pages) : 74 illustrations, 1 illustrations in color.
Subjects:
Medicine.
Virology.
Hepatology.
Infectious diseases.
Local subjects:
Medicine & Public Health.
Hepatology.
Infectious Diseases.
Virology.
System Details:
text file PDF
Summary:
With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.
Contents:
Detection and Quantification of HCV
Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays
Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen
Immunohistochemical Detection of HCV Proteins in Liver Tissue
HCV Nomenclature, Genotyping, and Quasispecies
Nomenclature and Numbering of the Hepatitis C Virus
A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates
Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry
HCV Replication in Vitro
Purification and Crystallization of NS5A Domain I of Hepatitis C Virus
NS5A Phosphorylation and Hyperphosphorylation
Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies
Structural and Functional Analysis of the HCV p7 Protein
HCV Replicons: Overview and Basic Protocols
Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA
Studying HCV RNA Synthesis In Vitro with Replication Complexes
Proteomics Study of the Hepatitis C Virus Replication Complex
Investigation of the Hepatitis C Virus Replication Complex
Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
High-Throughput Screening of HCV RNA Replication Inhibitors by Means of a Reporter Replicon System
Inhibition of HCV Replication by Small Interfering RNA
HCV Entry and Infection
Cellular Receptors and HCV Entry
Studying HCV Cell Entry with HCV Pseudoparticles (HCVpp)
Screening of Small-Molecule Compounds as Inhibitors of HCV Entry
Isolation of JFH-1 Strain and Development of an HCV Infection System
Measuring HCV Infectivity Produced in Cell Culture and In Vivo
Genotype 1a HCV (H77S) Infection System
Full-Length Infectious HCV Chimeras
Adaptation of the Hepatitis C Virus to Cell Culture
Primary Human Hepatocyte Culture for HCV Study
In Vivo Study of HCV in Mice with Chimeric Human Livers
Immunity and Modeling
Determination of HCV-Specific T-Cell Activity
Analysis of HCV-Specific T Cells by Flow Cytometry
Detection of Neutralizing Antibodies with HCV Pseudoparticles (HCVpp)
Mathematical Modeling of HCV Infection and Treatment.
Contributor:
Tang, Hengli, editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781597453943
Publisher Number:
10.1007/978-1-59745-394-3 doi
Access Restriction:
Restricted for use by site license.
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