Franklin

The Complement System [electronic resource] : Methods and Protocols / edited by Mihaela Gadjeva.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1100
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1100
Format/Description:
Book
1 online resource (XVI, 383 pages) : 81 illustrations, 27 illustrations in color.
Subjects:
Medicine.
Immunology.
Immunoglobulins.
Local subjects:
Biomedicine.
Immunology.
Antibodies.
System Details:
text file PDF
Summary:
Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established "classical" assays and recently developed "new" assays to analyze the complement system activation will be useful to a wide audience of scientists.
Contents:
Overview
Evaluation of Complement Function by ELISA
Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization
Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization
Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Purification and Characterisation of human and Mouse Complement C3
Purification of Human Complement Protein C5
Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
Purification and Functional Analysis of Human Properdin
Purification and Functional Characterization of C4b-binding Protein (C4BP)
Purification and Functional Characterization of Factor I
C1 inhibitor - Quantification and Purification
Purification, Quantification and Functional Analysis of Complement Factor H
Factor H-related Proteins
Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
Anti-Factor H Auto Antibodies Assay
Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Functional Analysis of C5a Effector Responses In Vitro and In Vivo
Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
Purification of C1q Receptors and Functional Analysis
Detection of Cell Membrane-bound CD46 using Flow Cytometry
Detection of CD59 using Flow Cytometry
Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
Analysis of the Complement Activation in Mice
Opsonophagocytic Assay.
Contributor:
Gadjeva, Mihaela. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781627037242
Publisher Number:
10.1007/978-1-62703-724-2 doi
Access Restriction:
Restricted for use by site license.
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