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Peptidomics [electronic resource] : Methods and Protocols / edited by Mikhail Soloviev.

Publication:
Totowa, NJ : Humana Press, 2010.
Format/Description:
Book
1 online resource (XIV, 396 pages)
Edition:
1st.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 615
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 615
Contained In:
Springer eBooks
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Subjects:
Life sciences.
Analytical chemistry.
Proteomics.
Proteins.
Cytology.
Local subjects:
Life Sciences. (search)
Cell Biology. (search)
Protein Science. (search)
Analytical Chemistry. (search)
Proteomics. (search)
System Details:
text file PDF
Summary:
Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
Contents:
Peptidomics: Divide et Impera
From Bacteria to Men
Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions
Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans
Mass Spectrometric Analysis of Molluscan Neuropeptides
Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry
Protocols for Peptidomic Analysis of Spider Venoms
Purification and Characterization of Biologically ctive Peptides from Spider Venoms
MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides
Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
Peptidomic Analysis of Single Identified Neurons
Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions
An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs
Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides
Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues
Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation
Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mass Spectrometric Analysis
Quantitative Neuroproteomics of the Synapse
Peptidomics Analysis of Lymphoblastoid Cell Lines
Peptidomics: Identification of Pathogenic and Marker Peptides
Tools and Approaches
Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra
Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors
Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays
In Situ Biosynthesis of Peptide Arrays
Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors
Bioinformatic Identification of Plant Peptides.
Contributor:
Soloviev, Mikhail. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781607615354
Publisher Number:
10.1007/978-1-60761-535-4 doi
Access Restriction:
Restricted for use by site license.