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DNA and RNA Profiling in Human Blood [electronic resource] : Methods and Protocols / edited by Peter Bugert.

Publication:
Totowa, NJ : Humana Press, 2009.
Format/Description:
Book
1 online resource (XI, 341 pages) : 63 illustrations, 1 illustrations in color.
Series:
METHODS IN MOLECULAR BIOLOGY™, 1064-3745 ; 496
Springer Protocols (Springer-12345)
METHODS IN MOLECULAR BIOLOGY™, 1064-3745 ; 496
Contained In:
Springer eBooks
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Subjects:
Medicine.
Human genetics.
Local subjects:
Biomedicine. (search)
Human Genetics. (search)
System Details:
text file PDF
Summary:
Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.
Contents:
DNA Profiling for Blood Cell Antigens
PCR-ELISA for High-Throughput Blood Group Genotyping
Single Base Extension in Multiplex Blood Group Genotyping
Real-Time PCR Assays for High-Throughput Blood Group Genotyping
Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing
Multiplex ABO Genotyping by Minisequencing
Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays
Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance
Multiplex HLA-Typing by Pyrosequencing
High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach
Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads
The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping
RNA Profiling in Blood Cells
Nanoliter High-Throughput PCR for DNA and RNA Profiling
RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis
RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR
cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR
Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE)
Genome-Wide Platelet RNA Profiling in Clinical Samples
PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples
MicroRNA Profiling of Megakaryocytes
Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes
Real-Time PCR Analysis for Blood Cell Lineage Specific Markers
Monitoring the Immune Response Using Real-Time PCR.
Contributor:
Bugert, Peter. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781597455534
Publisher Number:
10.1007/978-1-59745-553-4 doi
Access Restriction:
Restricted for use by site license.