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Molecular Biomethods Handbook [electronic resource] / edited by Ralph Rapley, John M. Walker.

Publication:
Totowa, NJ : Humana Press, 1998.
Series:
Springer Protocols (Springer-12345)
Format/Description:
Book
1 online resource (XIII, 725 pages) : 360 illustrations.
Subjects:
Life sciences.
Cytology.
Local subjects:
Life Sciences. (search)
Cell Biology. (search)
System Details:
text file PDF
Summary:
In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.
Contents:
Extraction of Total RNA from Tissues and Cultured Cells
Isolation of Total Cellular DNA from Tissues and Cultured Cells
Gel Electrophoresis of DNA
Sl Nuclease Analysis of RNA
Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)
Gene Probes
Nonradioactive Labeling of DNA
Southern Blot Analysis
Northern Blot Analysis
DNA Sequencing
Autoradiography and Fluorography
Mobility Shift Assays
cDNA Libraries
Genomic DNA Libraries
? as a Cloning Vector
Plasmid-Derived Cloning Vectors
M13 and Phagemid-Based Cloning Vectors
Retroviral Vectors From Laboratory Tools to Molecular Medicines
Baculovirus Vectors
Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
Plant Transformation
Restriction Fragment-Length Polymorphisms
Genome Mapping
Yeast Artificial Chromosomes
Polymerase Chain Reaction
In Vitro Transcriptions
In Vitro Translation
Site-Directed Mutagenesis
Site-Directed Mutagenesis
DNA Profiling
Radiolabeling of Peptides and Proteins
Protein Electrophoresis
Free Zone Capillary Electrophoresis
Protein Blotting
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Hydrophobic Interaction Chromatography
Affinity Chromatography
Reversed-Phase HPLC
Glycoprotein Analysis
Protein Sequencing
Solid-Phase Peptide Synthesis
Protein Engineering
Monoclonal Antibodies
Phage-Display Libraries
Enzyme- Linked Immunosorbent Assay (ELISA)
Epitope Mapping
lmmunocytochemistry
Flow Cytometry
Mass Spectrometry
The Technique of In Situ Hybridization.
Contributor:
Rapley, Ralph. editor.
Walker, John M. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781592596423
Publisher Number:
10.1007/978-1-59259-642-3 doi
Access Restriction:
Restricted for use by site license.
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