Molecular Biomethods Handbook [electronic resource] / edited by Ralph Rapley, John M. Walker.
- Totowa, NJ : Humana Press, 1998.
- Springer Protocols (Springer-12345)
1 online resource (XIII, 725 pages) : 360 illustrations.
- Life sciences.
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- In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.
- Extraction of Total RNA from Tissues and Cultured Cells
Isolation of Total Cellular DNA from Tissues and Cultured Cells
Gel Electrophoresis of DNA
Sl Nuclease Analysis of RNA
Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)
Nonradioactive Labeling of DNA
Southern Blot Analysis
Northern Blot Analysis
Autoradiography and Fluorography
Mobility Shift Assays
Genomic DNA Libraries
? as a Cloning Vector
Plasmid-Derived Cloning Vectors
M13 and Phagemid-Based Cloning Vectors
Retroviral Vectors From Laboratory Tools to Molecular Medicines
Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
Restriction Fragment-Length Polymorphisms
Yeast Artificial Chromosomes
Polymerase Chain Reaction
In Vitro Transcriptions
In Vitro Translation
Radiolabeling of Peptides and Proteins
Free Zone Capillary Electrophoresis
Hydrophobic Interaction Chromatography
Solid-Phase Peptide Synthesis
Enzyme- Linked Immunosorbent Assay (ELISA)
The Technique of In Situ Hybridization.
- Rapley, Ralph. editor.
Walker, John M. editor.
SpringerLink (Online service)
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- Publisher Number:
- 10.1007/978-1-59259-642-3 doi
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- Restricted for use by site license.
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