Franklin

Histology Protocols [electronic resource] / edited by Tim D. Hewitson, Ian A. Darby.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2010.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 611
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 611
Format/Description:
Book
1 online resource (X, 230 pages)
Subjects:
Life sciences.
Diagnosis, Laboratory.
Biochemistry.
Cytology.
Local subjects:
Life Sciences.
Cell Biology.
Biochemistry, general.
Laboratory Medicine.
System Details:
text file PDF
Summary:
With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.
Contents:
Tissue Processing
Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy
An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus
Staining Techniques
Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells
Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections
High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy
Lectin Histochemistry for Light and Electron Microscopy
Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain
Special Stains for Extracellular Matrix
Active Staining of Mouse Embryos for Magnetic Resonance Microscopy
Immunohistochemical Detection of Tumour Hypoxia
In Situ Localization of Apoptosis Using TUNEL
Imaging Techniques
Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord
High-Resolution Confocal Imaging in Tissue
Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography
Image Analysis and Quantitative Morphology.
Contributor:
Hewitson, Tim D. editor.
Darby, Ian A. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781603273459
Publisher Number:
10.1007/978-1-60327-345-9 doi
Access Restriction:
Restricted for use by site license.
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