Animal Cell Biotechnology [electronic resource] : Methods and Protocols / edited by Ralf Pörtner.

Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.
1 online resource (XIV, 498 pages) : 83 illustrations, 28 illustrations in color.
3rd ed. 2014.
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1104
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1104
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Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter
Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology
Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine
Scalable Transient Protein Expression
DNA Profiling and Characterisation of Animal Cell Lines
Tools for High-Throughput Process and Medium Optimization
Designing Media for Animal Cell Culture - CHO Cells, The Industrial Standard
Feed Optimization in Fed-Batch Culture
Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches
Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins
Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System
Monitoring Cell Growth, Viability and Apoptosis
Metabolic Flux Estimation in Mammalian Cell Cultures
Quenching Methods for the Analysis of Intracellular Metabolites
NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors
1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells
Microfluidic Perfusion Culture
Disposable Bioreactors for Inoculum Production and Protein Expression
Configuration of Bioreactors
Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes - Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)
Cell Assessment by Atline Microscopy
Seed Train Optimization for Cell Culture
Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines
Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles
Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology
Virus Separation Using Membranes.
Pörtner, Ralf. editor.
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