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Lipid Signaling Protocols [electronic resource] / edited by Banafshé Larijani, Rudiger Woscholski, Colin A. Rosser.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
Format/Description:
Book
1 online resource (XX, 429 pages)
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 462
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 462
Contained In:
Springer eBooks
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Subjects:
Life sciences.
Lipids.
Cytology.
Local subjects:
Life Sciences. (search)
Lipidology. (search)
Cell Biology. (search)
System Details:
text file PDF
Summary:
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology.
Contents:
Lipid Systems and Techniques
Eicosanoids: Generation and Detection in Mammalian Cells
Lipidomic Analysis of Phospholipids and Related Structures by Liquid Chromatography-Mass Spectrometry
Measurement of Polyphosphoinositides in Cultured Mammalian Cells
Inositol Lipid-Dependent Functions in : Analysis of Phosphatidylinositol Phosphates
Methods for the Determination of the Mass of Nuclear PtdIns4, PtdIns5, and PtdIns(4,5)
Lipid Quantification and Structure Determination of Nuclear Envelope Precursor Membranes in the Sea Urchin
Solution and Solid-State NMR of Lipids
Spatial Localization of PtdIns in Phase-Separated Giant Unilamellar Vesicles with a Fluorescent PLC-delta 1 PH Domain
Studying Cell-to-Cell Interactions: An Easy Method of Tethering Ligands on Artificial Membranes
Ceramide-Induced Transbilayer (Flip-Flop) Lipid Movement in Membranes
Effects of Sterols on the Development and Aging of
Proteomic Analysis of the Sterol-Mediated Signaling Pathway in
Lipid Metabolism and Recognition
Studying the Subcellular Localization and DNA-Binding Activity of FoxO Transcription Factors, Downstream Effectors of PI3K/Akt
Measurement of PTEN Activity by Imaging Phosphorylated Akt
Analysis of Phosphatidylinositol 3,4,5 Trisphosphate 5-Phosphatase Activity by and Assays
Measuring Phospholipase D Activity in Insulin-Secreting Pancreatic #x03B2;-Cells and Insulin-Responsive Muscle Cells and Adipocytes
Protein Kinase C as an Effector of Lipid-Derived Second Messengers
Detection of Myotubularin Phosphatases Activity on Phosphoinositides and
Quantification of Multiple Phosphatidylinositol 4-Kinase Isozyme Activities in Cell Extracts
Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy
Revealing Signaling in Single Cells by Single- and Two-Photon Fluorescence Lifetime Imaging Microscopy
Phosphoinositide (PI) 3-Kinase Assays
Measurement of Phosphatidylinositol and Phosphatidylcholine Binding and Transfer Activity of the Lipid Transport Protein PITP
Reconstitution of Activation of PLC#x03B5; by Ras and Rho GTPases
Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities
Preparation of Membrane Rafts.
Contributor:
Larijani, Banafshé. editor.
Woscholski, Rudiger. editor.
Rosser, Colin A. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781603271158
Publisher Number:
10.1007/978-1-60327-115-8 doi
Access Restriction:
Restricted for use by site license.