Apoptosis Methods and Protocols [electronic resource] / edited by Hugh J. M. Brady.

Totowa, NJ : Humana Press, 2004.
1 online resource (XI, 304 pages) : 74 illustrations.
Methods in molecular biology 1064-3745 ; 282
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 282
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The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
Measurement of Apoptosis by DNA Fragmentation
Measurement of Caspase Activity in Cells Undergoing Apoptosis
Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells
Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays
Detecting and Quantifying Apoptosis in Tissue Sections
Immunoassay for Single-Stranded DNA in Apoptotic Cells
Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptosis
Measurement of Changes in Intracellular Calcium During Apoptosis
Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
Assays to Measure Stress-Activated MAPK Activity
Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability
Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
Measuring Programmed Cell Death in Plants
Detection of Apoptosis in Drosophila
Measurement of Apoptotic Cell Clearance In Vitro
Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20
The Yeast Three-Hybrid System As a Tool to Study Caspases
Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA
Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis.
Brady, Hugh J. M. editor.
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