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Bacterial Chromatin [electronic resource] : Methods and Protocols / edited by Remus T. Dame.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Format/Description:
Book
1 online resource (XI, 419 pages) : 118 illustrations, 81 illustrations in color.
Series:
Methods in molecular biology 1064-3745 ; 1837
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1837
Contained In:
Springer eBooks
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Subjects:
Life sciences.
Microbiology.
Local subjects:
Life Sciences. (search)
Microbiology. (search)
System Details:
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Summary:
This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Chromatin: Methods and Protocols aims to be useful as an up-to-date reference work for scholars in the bacterial chromatin field, those entering the field from adjacent research fields, and scientists in the eukaryotic chromatin field.
Contents:
Determination of the 3D Genome Organization of Bacteria using Hi-C
Overview of Processing and Analyzing Hi-C Data on Bacteria
GeF-seq:A Simple Procedure for Base Pair Resolution ChIP-seq
Genomic SELEX Screening of Regulatory Targets of Escherichia coli Transcription Factors
Modular assembly of Synthetic Secondary Chromosomes
High-resolution Characterization of DNA/Protein Complexes in Living Bacteria
Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-dimensional Superresolution Structured Illumination Microscopy
Genetic Approaches to Study the Interplay Between Transcription and Nucleoid-associated Proteins in Escherichia coliAtomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organization
Dynamic Light Scattering of DNA-ligand Complexes
Microscale Thermophoresis Analysis of Chromatin Interactions
Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins
Approaches for Determining DNA Persistence Length using Atomic Force Microscopy
Quantitation of DNA Binding Affinity using Tethered Particle Motion
Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-stretching with Single-molecule Imaging
Unravelling the Biophysical Properties of Chromatin Proteins and DNA using Acoustic Force Spectroscopy
Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers
In Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase
Deciphering 3D Organization Of Chromosomes using Hi-C Data
Molecular Dynamics Simulation of a Feather-boa Model of Bacterial Chromosome. .
Contributor:
Dame, Remus T. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781493986750
Publisher Number:
10.1007/978-1-4939-8675-0 doi
Access Restriction:
Restricted for use by site license.