Franklin

Wnt Signaling [electronic resource] / edited by Elizabeth Vincan.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
Series:
Methods in Molecular Biology, 1064-3745 ; 469
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 469
Format/Description:
Book
1 online resource (XVI, 494 pages)
Subjects:
Life sciences.
Cytology.
Local subjects:
Life Sciences.
Cell Biology.
System Details:
text file PDF
Summary:
Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource. .
Contents:
Evolution of the Wnt Pathways
Dictyostelium
Dictyostelium Development: A Prototypic Wnt Pathway?
Monitoring Patterns of Gene Expression in Dictyostelium by ?-galacotsidase Staining
Use of the Dictyostelium Stalk Cell Assay to Monitor GSK-3 Regulation
Cnidarians
Wnt Signaling in Cnidarians
Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos
Detection of Expression Patterns in Hydra Pattern Formation
C. Elegans
Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
Wnt Signaling During Caenorhabditis elegans Embryonic Development
Drosophila
Function of the Wingless Signaling Pathway in Drosophila
Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster
Wingless Signaling in Drosophila Eye Development
High-Throughput RNAi Screen in Drosophila
Sea Urchin
Wnt Signaling in the Early Sea Urchin Embryo
Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos
Functional Analysis of Wnt Signaling in the Early Sea Urchin Embryo Using mRNA Microinjection
Zebrafish
Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish
Determination of mRNA and Protein Expression Patterns in Zebrafish
Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches
Neural Patterning and CNS Functions of Wnt in Zebrafish
Xenopus
Studying Wnt Signaling in Xenopus
Analysis of Gene Expression in Xenopus Embryos
Detection of Nuclear ?-catenin in Xenopus Embryos
Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus
Gain-of-Function and Loss-of-Function Strategies in Xenopus
How The Mother Can Help: Studying Maternal Wnt Signaling by Anti-sense-mediated Depletion of Maternal mRNAs and the Host Transfer Technique
Inducible Gene Expression in Transient Transgenic Xenopus Embryos
Wnt-Frizzled Interactions in Xenopus
Dorsal Axis Duplication as a Functional Readout for Wnt Activity
Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo
Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula.
Contributor:
Vincan, Elizabeth. editor.
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
ISBN:
9781603274692
Publisher Number:
10.1007/978-1-60327-469-2 doi
Access Restriction:
Restricted for use by site license.
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