Franklin

Epigenetic Methods in Neuroscience Research [electronic resource] / edited by Nina Karpova.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2016.
Format/Description:
Book
1 online resource (XII, 258 pages) : 43 illustrations, 37 illustrations in color.
Edition:
1st ed. 2016.
Series:
Neuromethods 0893-2336 ; 105
Springer Protocols (Springer-12345)
Neuromethods, 0893-2336 ; 105
Contained In:
Springer eBooks
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Subjects:
Neurosciences.
Local subjects:
Neurosciences. (search)
System Details:
text file PDF
Summary:
This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research  guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3'UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.    Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.
Contents:
Epigenetics: From Basic Biology To Chromatin Modifying Drugs And New Potential Clinical Applications
Analysis Of Brain Epigenome: A Guide To Epigenetic Methods
Analysis Of DNA Methylation By Bisulfite Reaction In Neural Cells As An Example Of Orexin Neurons
Protocol For The Simultaneous Analysis Of Gene DNA Methylation And Mrna Expression Levels In The Rodent Brain
Simultaneous Quantification Of Global 5mc And 5hmc Levels In The Nervous System Using A HPLC/MS Method
Protocol For Methylated DNA Immunoprecipitation (Medip) Analysis
Cell Type-Specific DNA Methylation Analysis In Neurons And Glia
Immunohistochemical Detection Of Oxidized Forms Of 5-Methylcytosine In Embryonic And Adult Brain Tissue
Analysis Of Histone Modifications In Neural Cells As An Example Of Orexin Neurons
Site-Specific Delivery Of Epigenetic Modulating Drugs Into The Rat Brain
Mammalian Genome Plasticity: Expression Analysis Of Transposable Elements
Tools For Analyzing The Role Of Local Protein Synthesis In Synaptic Plasticity
In Situ Detection Of Neuron-Specific Micrornas In Frozen Brain Tissue Meister
Protocol For High-Throughput Mirna Profiling Of The Rat Brain
Metabolites As Clock Hands: Estimation Of Internal Body Time Using Blood Metabolomics.
Contributor:
Karpova, Nina. editor., Editor,
SpringerLink (Online service)
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Printed edition:
Printed edition:
Printed edition:
ISBN:
9781493927548
Publisher Number:
10.1007/978-1-4939-2754-8 doi
Access Restriction:
Restricted for use by site license.