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Single-Cell Analysis [electronic resource] : Methods and Protocols / edited by Sara Lindström, Helene Andersson-Svahn.

Publication:
Totowa, NJ : Humana Press, 2012.
Format/Description:
Book
1 online resource (X, 238 pages) : 63 illustrations, 16 illustrations in color.
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 853
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 853
Contained In:
Springer eBooks
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Subjects:
Life sciences.
Cytology.
Cell physiology.
Local subjects:
Life Sciences. (search)
Cell Biology. (search)
Cell Physiology. (search)
System Details:
text file PDF
Summary:
Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail.  The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.
Contents:
Introduction: Why Analyze Single Cells?
Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview
Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry
Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts
Single-Cell Culture in Microwells
Iso-Dielectric Separation and Analysis of Cells
Single Cell Electroporation Using Microfluidic Devices
Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array
Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
Droplet Microfluidics for Single-Cell Analysis
Screening of Antigen-Specific Antibody Secreting Cells
Analysis of Single Eukaryotic Cells Using Raman Tweezers
Single-Cell Microinjection Technologies
Ultrasonic Manipulation of Single Cells
Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices
Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses.
Contributor:
Lindström, Sara, editor.
Andersson-Svahn, Helene. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781617795671
Publisher Number:
10.1007/978-1-61779-567-1 doi
Access Restriction:
Restricted for use by site license.