Rho GTPases [electronic resource] : Methods and Protocols / edited by Francisco Rivero.

New York, NY : Springer New York, 2012.
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 827
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 827
1 online resource (XIV, 407 pages) : 78 illustrations, 2 illustrations in color.
Life sciences.
Chemistry, Analytic.
Local subjects:
Life Sciences.
Biochemistry, general.
Analytical Chemistry.
System Details:
text file PDF
Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few. In addition, Rho GTPases have acquired medical relevance because of their participation in tumorigenesis and metastasis, in cardiovascular conditions, and as targets of infectious agents. The field has broadened even more with the contribution of studies in model organisms (plants, amoebas, fungi, invertebrates), each adding a particular view to the complexity of the family and vastly enriching our perception of these important signaling components. Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts. Additionally, the volume devotes a section to advanced imaging methods and to recently developed high throughput methods, closing with techniques specifically designed for studies in selected non-mammalian model organisms. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Rho GTPase: Methods and Protocols provides techniques that are standard for researchers in the field but also includes protocols for those who, being already familiar with some of the techniques, wish to explore additional aspects.
Historical Overview of Rho GTPases
Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family
Biochemical Assays to Characterize Rho GTPases
How to Analyze Bacterial Toxins Targeting Rho GTPases
Assessing Ubiquitylation of Rho GTPases in Mammalian Cells
Posttranslational Lipid Modification of Rho Family Small GTPases
Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of Rho GTPases
A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases
Rho GTPases and Cancer Cell Transendothelial Migration
Quantitative and Robust Assay to Measure Cell-cell Contact Assembly and Maintenance
Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice
Rho GTPase Techniques in Osteoclastogenesis
Assessment of Rho GTPase Signaling During Neurite Outgrowth
Assesment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Multiplex Imaging of Rho GTPase Activities in Living Cells
FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor Mediated Phagocytosis in Macrophages
High Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors
Functional Analysis of RhoGTPase Activation and Inhibition in a Bead-Based Miniaturized Format
Use of Phage Display For the Identification of Molecular Sensors Specific for Activated Rho
Identification of New Interacting Partners for Atypical Rho-GTPases: a SILAC-Based Approach
Using Zebrafish for Studying Rho GTPases Signaling in Vivo
Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
Protocol for ex vivo Incubation of Drosophila Primary Post-Embryonic Haemocytes for Real-Time Analyses
Analysis of Rho GTPase Activation in Saccharomyces cerevisiae
Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes
Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes.
Rivero, Francisco. editor.
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10.1007/978-1-61779-442-1 doi
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