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Hepatocyte Transplantation [electronic resource] : Methods and Protocols / edited by Anil Dhawan, Robin D. Hughes.

Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
Format/Description:
Book
1 online resource (XVII, 231 pages)
Series:
Methods in molecular biology 1064-3745 ; 481
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 481
Contained In:
Springer eBooks
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Subjects:
Life sciences.
Biochemistry.
Cytology.
Stem cells.
Local subjects:
Life Sciences. (search)
Biochemistry, general. (search)
Cell Biology. (search)
Stem Cells. (search)
System Details:
text file PDF
Summary:
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Contents:
Human Hepatocyte Transplantation Overview
Isolation of Human Hepatocytes
An Optimised Method for Cryopreservation of Human Hepatocytes
Liver Cell Culture Techniques
In vitro Assays for Induction of Drug Metabolism
Hepatocyte Apoptosis
Small Animal Models of Hepatocyte Transplantation
Hepatocyte Transplantation Techniques: Large Animal Models
Cell Transplant Techniques: Engraftment Detection of Cells
Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation
Ex vivo Gene Transfer into Hepatocytes
Sources of Adult Hepatic Stem Cells: Haematopoietic
Production of Hepatocyte-like Cells from Human Amnion
Generation of Hepatocytes from Human Embryonic Stem Cells
Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells
Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro
Liver Cell Labelling with MRI Contrast Agents
Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory.
Contributor:
Dhawan, Anil. editor.
Hughes, Robin D. editor.
SpringerLink (Online service)
Other format:
Printed edition:
ISBN:
9781597452014
Publisher Number:
10.1007/978-1-59745-201-4 doi
Access Restriction:
Restricted for use by site license.