Franklin

Microinjection [electronic resource] : Methods and Protocols / edited by Chengyu Liu, Yubin Du.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2019.
Series:
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1874
Methods in Molecular Biology, 1064-3745 ; 1874
Format/Description:
Book
1 online resource (XIV, 540 pages) : 134 illustrations, 96 illustrations in color.
Subjects:
Biotechnology.
Human genetics.
Local subjects:
Biotechnology.
Human Genetics.
System Details:
text file PDF
Summary:
This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
Contents:
Microinjection and Micromanipulation: A Historical Perspective
Production of Transgenic Mice by Pronuclear Microinjection
Transgene Recombineering in Bacterial Artificial Chromosomes
Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice
Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection
Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
Organ Generation from Knocked-Out Rat Blastocysts Complemented with Pluripotent Stem Cells
Generation of Rabbit Models by Gene Editing Nucleases
Production of Genetically Engineered Porcine Embryos by Handmade Cloning
Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production
Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer
Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting
Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes
Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing
Microinjection in Zebrafish for Genome Editing and Functional Studies
Microinjection of Marine Fish Eggs
Generating Gene Knockout Oryzias Latipes and Rice Field Eel Using TALENs Method
Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus
Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function.
Contributor:
Liu, Chengyu. editor., Editor,
Du, Yubin. editor., Editor,
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
Printed edition:
ISBN:
9781493988310
Publisher Number:
10.1007/978-1-4939-8831-0 doi
Access Restriction:
Restricted for use by site license.
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