Franklin

Phytoplasmas [electronic resource] : Methods and Protocols / edited by Rita Musetti, Laura Pagliari.

Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2019.
Series:
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1875
Methods in Molecular Biology, 1064-3745 ; 1875
Format/Description:
Book
1 online resource (XI, 362 pages) : 75 illustrations, 64 illustrations in color.
Subjects:
Botany.
Local subjects:
Plant Sciences. (search)
System Details:
text file PDF
Summary:
This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
Phytoplasmas: An Introduction
Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques
Phytoplasma Transmission: Insect Rearing and Infection Protocols
Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors
Symptoms of Phytoplasma Diseases
Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas
Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene
PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas
Real-time PCR Protocol for Phytoplasma Detection and Quantification
Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves
A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas
One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas
A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification
Quantitative Analysis with Droplet Digital PCR
Rapid Sample Preparation and LAMP for Phytoplasma Detection
Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly
Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of 'Flavescence Dorée'
Transcriptomic Analyses of Phytoplasmas
Transcriptomic Analyses of Phytoplasmas
Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study
Collection of Phloem Sap in Phytoplasma-infected Plants
DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas
Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas
Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC)
Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants
Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants. .
Contributor:
Musetti, Rita. editor., Editor,
Pagliari, Laura. editor., Editor,
SpringerLink (Online service)
Contained In:
Springer eBooks
Other format:
Printed edition:
Printed edition:
ISBN:
9781493988372
Publisher Number:
10.1007/978-1-4939-8837-2 doi
Access Restriction:
Restricted for use by site license.
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