Odontogenesis : methods and protocols / edited by Petros Papagerakis.

New York, NY : Humana Press, [2019]
xviii, 565 pages : illustrations (some color) ; 27 cm.
Methods in molecular biology (Clifton, N.J.) ; v. 1922.
Springer protocols (Series)
Methods in molecular biology, 1064-3745 ; 1922
Springer protocols

Get It


Teeth -- Growth -- Laboratory manuals.
Medical subjects:
Tooth -- growth & development.
Microdissection and isolation of mouse dental epithelial cells of continuously growing mouse incisors / Hidemitsu Harada and Keishi Otsu
Establishment of an immortalized mouse bmp2 knockout dental papilla mesenchymal cell line / Wen'an Xu and Shuo Chen
Establishment of stable cell lines from primary human dental pulp stem cells / Elizabeth Guirado, Youbin Zhang, and Anne George
Isolation of dental stem cell-enriched populations from continuously growing mouse incisors / Anamaria Balic
Application of cell lineage tracing combined with immunofluorescence in the study of dentinogenesis / Yan Jing, Chaoyuan Li, and Jian Q. Feng
Tissue recombination and kidney capsule transplantation assays for the study of epithelial-mesenchymal interactions / Lucia Jimenez-Rojo and Thimios A. Mitsiadis
Dental mesenchymal stem cells : dental pulp stem cells, periodontal ligament stem cells, apical papilla stem cells, and primary teeth stem cells-isolation, characterization, and expansion for tissue engineering / Mey Al-Habib and George T. -J. Huang
Phenotypic identification of dental pulp mesenchymal stem/stromal cells subpopulations with multiparametric flow cytometry / Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Fre´de´ric Mallein-Gerin, and Hugo Fabre
Dental pulp stem cells: isolation, characterization, expansion, and odontoblast differentiation for tissue engineering / Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng, Silvana Papagerakis, Daniel Chen, and Petros Papagerakis
In vitro analysis of intramolecular signaling events in PDLSCs using confocal and TIRF microscopy / Annette Merkel and Anne George
Mouse model to study reparative dentinogenesis / R. C. Babb, D. Chandrasekaran, L. K. Zaugg, and P. T. Sharpe
Multiwalled carbon nanotubes for dental applications / Petros Kechagioglou, Eleftherios Andriotis, Petros Papagerakis, and Silvana Papagerakis
Peptide-mediated biomimetic regrowth of human enamel in situ / Kaushik Mukherjee, Qichao Ruan, and Janet Moradian-Oldak
Bioengineering tooth bud constructs using GelMA hydrogel / Elizabeth E. Smith and Pamela C. Yelick
Whole-mount in situ hybridization of mouse embryos using DIG-labeled RNA probes / Jingyi Wu and Xiaofang Wang
In situ hybridization on mouse paraffin sections using DIG-labeled RNA probes / Jingyi Wu, Jian Q. Feng, and Xiaofang Wang
Methods for in situ protein visualization in dental mineralized tissues / D. Hotton, A. Berdal, and A. Bolanos
In situ hybridization in mineralized tissues : the added value of LNA probes for RNA detection / G. Lignon, D. Hotton, A. Berdal, and A. Bolanos
Immunofluorescence procedure for developing enamel tissues / Xu Yang and Elia Beniash
Silver-albumin tissue staining protocol to visualize odontogenesis in whole embryos / Julia C. Boughner and David M. L. Cooper
Isolation of SIBLING proteins from bone and dentin matrices / Jingyi Wu and Xiaofang Wang
Immunohistochemical co-localization of amelogenin and ameloblastin in developing enamel matrix / Rucha Arun Bapat and Janet Moradian-Oldak
Expression and purification of recombinant mouse ameloblastin in E. coli / Jingtan Su, Rucha Arun Bapat, and Janet Moradian-Oldak
Protocols for Studying formation and mineralization of dental tissues in vivo : extraction protocol for isolating dentin matrix proteins from developing teeth / Yasuo Yamakoshi, Jan C.-C. Hu, Mari M. Saito, and James P. Simmer
Purification of Developing enamel matrix proteins using preparative SDS-PAGE / Steven J. Brookes and Claire M. Gabe
Using imageJ (FIJI) to analyze and present x-ray CT images of enamel / Steven J. Brookes
Scanning electron microscopy (SEM) methods for dental enamel / Steinar Risnes, Muhammad Saeed, and Amer Sehic
Microcomputed tomography imaging in odontogenesis studies / Kostas Verdelis and Phil Salmon
Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) for the examination of dental hard tissues / Victor E. Arana-Chavez and Leticia S. Castro-Filice
Rodent dental fluorosis model: extraction of enamel organ from rat incisors / M. Suzuki and J. D. Bartlett
Three-dimensional assessment of crown size and eruption space for developing third molars : data collection techniques based on cone-beam computed tomography (CBCT) / D. F. Marchiori, G. V. Packota, and J. C. Boughner
Protocols to study dental caries in vitro : microbial caries models / Bennett T. Amaechi, Livia M. A. Tenuta, Antonio P. Ricomini Filho, and Jaime A. Cury
In vitro caries models for the assessment of novel restorative materials / Basma Sulaiman Ghandourah, Anna Lefkelidou, Raed Said, Xanthippi Chatzistavrou, Susan Flannagan, Carlos Gonza les-Cabezas, Christopher J. Fenno, Li Zheng, Silvana Papagerakis, and Petros Papagerakis
Protocols to study dental caries in vitro : pH cycling models / Bennett T. Amaechi
In vivo rodent models for studying dental caries and pulp disease / June Hsiao, Yuanyuan Wang, Li Zheng, Ruirui Liu, Raed Said, Lubomir Hadjiyski, Heekon Cha, Tatiana Botero, Xanthippi Chatzistavrou, Qing Dong, Silvana Papagerakis, and Petros Papagerakis
Protocol GenoDENT : implementation of a new NGS panel for molecular diagnosis of genetic disorders with orodental involvement / Tristan Rey, Julien Tarabeux, Benedicte Gerard, Marion Delbarre, Antony Le Bechec, Corinne Stoetzel, Megana Prasad, Virginie Laugel-Haushalter, Marzena Kawczynski, Jean Muller, Jamel Chelly, Helene Dollfus, Marie-Cecile Maniere, and Agnes Bloch-Zupan
Protocols for genetic and epigenetic studies of rare diseases affecting dental tissues / Bruna Rabelo Amorim, Pollyanna Almeida Costa dos Santos, Caroline Lourenco de Lima, Denise Carleto Andia, Juliana Forte Mazzeu, and Ana Carolina Acevedo
Protocols, Methods, and tools for genome-wide association studies (GWAS) of dental traits / Cary S. Agler, Dmitry Shungin, Andrea G. Ferreira Zandona, Paige Schmadeke, Patricia V. Basta, Jason Luo, John Cantrell, Thomas D. Pahel Jr., Beau D. Meyer, John R. Shaffer, Arne S. Schaefer, Kari E. North, and Kimon Divaris
Measurement of early childhood oral health for research purposes: dental caries experience and developmental defects of the enamel in the primary dentition / Jeannie Ginnis, Andrea G. Ferreira Zandona , Gary D. Slade, John Cantrell, Mikafui E. Antonio, Bhavna T. Pahel, Beau D. Meyer, Poojan Shrestha, Miguel A. Simancas-Pallares, Ashwini R. Joshi, and Kimon Divaris
Supragingival biofilm in early childhood caries : clinical and laboratory protocols and bioinformatics pipelines supporting metagenomics, metatranscriptomics, and metabolomics studies of the oral microbiome / Kimon Divaris, Dmitry Shungin, Adaris Rodrı´guez-Cortes, Patricia V. Basta, Jeff Roach, Hunyong Cho, Di Wu, Andrea G. Ferreira Zandona, Jeannie Ginnis, Sivapriya Ramamoorthy, Jason M. Kinchen, Jakub Kwintkiewicz, Natasha Butz, Apoena A. Ribeiro, and M. Andrea Azcarate-Peril
Saliva and gingival crevicular fluid (GCF) collection for biomarker screening / Petros Papagerakis, Li Zheng, Doohak Kim, Raed Said, Amber A. Ehlert, Kevin K. M. Chung, and Silvana Papagerakis.
Includes bibliographical references and index.
Papagerakis, Petros, editor.