Franklin

Mass Spectrometry of Proteins : Methods and Protocols / edited by Caroline A. Evans, Phillip C. Wright, Josselin Noirel.

Publication:
New York, NY : Springer New York : Imprint: Humana, 2019.
Format/Description:
Book
1 online resource (XIII, 264 pages) : 54 illustrations, 35 illustrations in color.
Edition:
1st ed. 2019.
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 1977
Methods in Molecular Biology, 1064-3745 ; 1977
Contained In:
Springer eBooks
Status/Location:
Loading...

Options
Location Notes Your Loan Policy

Details

Subjects:
Proteins.
Mass spectrometry.
Local subjects:
Protein Science. (search)
Mass Spectrometry. (search)
System Details:
text file PDF
Summary:
New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With applications to biomarker discovery, interactions between proteins, between biological systems, dynamics of post-translational modifications among others, new protocols have been developed and iteratively refined to probe the endless complexity of the proteome in ever greater details. This volume deals with methods for data dependent and data independent mass spectrometry analyses. Valuable, first-hand information is provided from designing experiments, sample preparation and analysis, exploitation of public datasets and carrying out reproducible data pipelines, using modern computational tools such as Galaxy or Jupyter. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry of Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
Contents:
Next Generation Proteomics for Clinical Biomarker Detection using SWATH-MS
A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification of Protein Ubiquitination
Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometry
Quantitative Phosphoproteomic using Titanium Dioxide Micro-Columns and Label Free Quantitation
Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond
Quantitative Analysis of Protein S-Acylation Site Dynamics Using Site-Specific Acyl-Biotin Exchange (ssABE)
Reducing Complexity? Cysteine Reduction and S-alkylation in Proteomic Workflows: Practical Considerations
Detection of Unknown Chemical Adduct Modifications on Proteins: From Wet to Dry Laboratory
Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry
Metaproteomics of Freshwater Microbial Communities
Peptide-to-Protein Summarization: An Important Step for Accurate Quantification In Label-Based Proteomics
Experimental Design in Quantitative Proteomics
Practical Integration of Multi-Run iTRAQ Data
Quantitative Proteomics Data in the Public Domain: Challenges and Opportunities
Computational Proteomics with Jupyter and Python
The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis.
Contributor:
Evans, Caroline A. editor., Editor,
Wright, Phillip C. editor., Editor,
Noirel, Josselin, editor., Editor,
SpringerLink (Online service)
Other format:
Printed edition:
Printed edition:
Printed edition:
ISBN:
978-1-4939-9232-4
9781493992324
Publisher Number:
10.1007/978-1-4939-9232-4 doi
Access Restriction:
Restricted for use by site license.