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Chromosome microdissection and cloning [electronic resource] : a practical guide / Nabil G. Hagig and Michael V. Viola.

Author/Creator:
Hagag, Nabil G.
Publication:
San Diego : Academic Press, 1993.
Format/Description:
Book
1 online resource (171 p.)
Status/Location:
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Subjects:
Molecular cloning -- Technique.
Microdissection.
Chromosomes.
Form/Genre:
Electronic books.
Language:
English
Summary:
Chromosome Microdissection And Cloning
Contents:
Front Cover; Chromosome Microdissection and Cloning: A Practical Guide; Copyright Page; Table of Contents; Preface; Chapter 1.Introduction to Chromosome Microdissection; Chromosome Organization; Cloning DNA from Chromosome Fragments; References; Chapter 2.Preparation of Chromosomes for Microdissection; Introduction; Critical Aspects of Chromosome Preparation; Reagents; Equipment; Protocols; References; Chapter 3.Methods of Chromosome Microdissection; Introduction; Methods; Summary of Chromosome Microdissection and Collection for DNA Cloning; References
Chapter 4.Molecular Cloning of Microdissected Chromosomal DNAIntroduction; Cloning DNA from Microdissected Chromosomal DNA Fragments; Method 1. Direct Cloning of DNA from Microdissected Chromosomal Fragments; Method 2. Ligation of Microdissected Chromosomal DNA with Plasmid Vector or Linker-Adaptor and PCR Amplification; Method 3. PCR Amplification of Microdissected Chromosomal DNA Fragments Followed by Probing a Complete Recombinant Library; Analysis of Recombinant Clones Derived from Microdissected Chromosomal DNA; Determination of DNA Insert Size Range
Determination of the Percentage of Recombinant Clones Containing Repeat and Unique SequencesCalculation of the Percentage of Total Microdissected DNA Cloned; Determination of Potential Structural Gene Sequences; Localization of Recombinant Clones Using in Situ Hybridization; References; Chapter 5.Applications of Chromosome Microdissection; Direct Analysis of the PCR Product of Microdissected Chromosome Fragments; Recombinant DNA Libraries Generated from Microdissected Chromosome Fragments; Gene Transfer Using Chromosome Fragments; References; Appendix: List of Suppliers and Addresses
GlossaryIndex
Notes:
Description based upon print version of record.
Includes bibliographical references and index.
Contributor:
Viola, Michael V.
ISBN:
0-323-16077-8
1-299-19368-4
OCLC:
829460439