Front Cover; Chromosome Microdissection and Cloning: A Practical Guide; Copyright Page; Table of Contents; Preface; Chapter 1.Introduction to Chromosome Microdissection; Chromosome Organization; Cloning DNA from Chromosome Fragments; References; Chapter 2.Preparation of Chromosomes for Microdissection; Introduction; Critical Aspects of Chromosome Preparation; Reagents; Equipment; Protocols; References; Chapter 3.Methods of Chromosome Microdissection; Introduction; Methods; Summary of Chromosome Microdissection and Collection for DNA Cloning; References Chapter 4.Molecular Cloning of Microdissected Chromosomal DNAIntroduction; Cloning DNA from Microdissected Chromosomal DNA Fragments; Method 1. Direct Cloning of DNA from Microdissected Chromosomal Fragments; Method 2. Ligation of Microdissected Chromosomal DNA with Plasmid Vector or Linker-Adaptor and PCR Amplification; Method 3. PCR Amplification of Microdissected Chromosomal DNA Fragments Followed by Probing a Complete Recombinant Library; Analysis of Recombinant Clones Derived from Microdissected Chromosomal DNA; Determination of DNA Insert Size Range Determination of the Percentage of Recombinant Clones Containing Repeat and Unique SequencesCalculation of the Percentage of Total Microdissected DNA Cloned; Determination of Potential Structural Gene Sequences; Localization of Recombinant Clones Using in Situ Hybridization; References; Chapter 5.Applications of Chromosome Microdissection; Direct Analysis of the PCR Product of Microdissected Chromosome Fragments; Recombinant DNA Libraries Generated from Microdissected Chromosome Fragments; Gene Transfer Using Chromosome Fragments; References; Appendix: List of Suppliers and Addresses GlossaryIndex
Description based upon print version of record. Includes bibliographical references and index.